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Immortalized-Human-Nasopharyngeal-Epithelial-Cells-(NP69SV40T)

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Immortalized-Human-Nasopharyngeal-Epithelial-Cells-(NP69SV40T)

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Immortalized-Human-Nasopharyngeal-Epithelial-Cells-(NP69SV40T)

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	Print Version
BioSafety Level	II
Organism	Homo sapiens
Source Organ	Nasopharynx
Growth Properties	Adherent
Morphology	Epithelial-like
Recommended Seeding Density	2 x 10⁴ cells/cm²
Applications	For Research Use Only
Immortalization Method	Transfected with the PX8 plasmid containing SV40T antigen
Description	Immortalized Human Nasopharyngeal Epithelial Cells (NP69SV40T) is derived from isolated primary non-diseased nasopharyngeal epithelial cells which were transfected with the PX8 plasmid containing SV40T antigen. The cells retained their primary characteristics and are non-tumorigenic. NP69SV40T cells are responsive to TGFβ1, exhibiting growth inhibition in the presence of the cytokine. These cells are a reliable source of nasopharyngeal epithelial cells for in vitro model for studies in the mechanisms of nasopharyngeal carcinoma tumorigenesis.
Procedure Overview
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Propagation	Use of PriCoat? T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The base medium for this cell line is PriGrow X Series, available from abm (TM0634). Atmosphere: air: 95%, CO?: 5%; Temperature: 37.0°C.
Quality Control	1) SV40T protein detected through Western blotting analysis; 2) TRAP assay was ultilized to determine telomerase activity.
Reference	Tsao, S. W., Wang, X., Liu, Y., Cheung, Y. C., Feng, H., Zheng, Z., . . . Huang, D. (2002). Establishment of two immortalized nasopharyngeal epithelial cell lines using SV40 large T and HPV16E6/E7 viral oncogenes. Biochimica Et Biophysica Acta (BBA) - Molecular Cell Research, 1590(1-3), 150-158. doi:10.1016/s0167-4889(02)00208-2
Disclaimer	1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.