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Immortalized-Equine-Lung-Cells-(extEqFL)

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Immortalized-Equine-Lung-Cells-(extEqFL)

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Immortalized-Equine-Lung-Cells-(extEqFL)

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	Print Version
BioSafety Level	II
Organism	5-month old Equine Fetus
Source Organ	Lung
Growth Properties	Adherent
Morphology	Polygonal
Population Doubling	72 hours
Recommended Seeding Density	5x10⁴ cells/cm²
Applications	For Research Use Only
Immortalization Method	Infected with non-replicating retrovirus containing a supernatant from the PA317 LXSN HPV16E6E7
Description	Immortalized Equine Lung Cells (extEqFL) are immortalized with the HPV16 E6/E7 gene through infection with non-replicating retrovirus carrying the PA317 LXSN HPV16 E6/E7 vector. These cells are a reliable tool for research in equine lung biology and can be used to assess the effects of equine related viruses. The extEqFL cells in vitro can be genetically manipulated for further research.
Procedure Overview
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Propagation	Use of PriCoat? T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The base medium for this cell line is Prigrow III medium available from ABM (TM003). To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999) to final concentration of 5%, 1% L-glutamine (G275), 600 μg/ml G418 (G271), and Penicillin/Streptomycin (G255). Atmosphere: air: 95%, CO?: 5%; Temperature: 37.0°C.
Quality Control	1) Selected cells with presence of 600 μg/ml G418, 2) PCR analysis for HPV16 E6/E7 presence.
Disclaimer	1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.