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Immortalized-Mouse-Sertoli-Cells-(ST38c)

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Immortalized-Mouse-Sertoli-Cells-(ST38c)

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Immortalized-Mouse-Sertoli-Cells-(ST38c)

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	Print Version
BioSafety Level	II
Organism	Mouse
Source Organ	Testes
Donor Gender	Male
Growth Properties	Adherent
Morphology	Spindle-shaped
Recommended Seeding Density	Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Applications	For Research Use Only
Immortalization Method	Isolated from a transgenic mouse carrying SV40 large T Antigen under the control of the human vimentin promoter
Description	Previously immortalized sertoli cells- involved in androgen regulation during spermatogenesis- lose androgen receptor (AR) expression or androgen responsiveness; thus leaving them ill-suited for any further research regarding androgen regulation. The Immortalized Mouse Sertoli Cells (ST38c) eliminate this barrier with their continuous expression of endogenous AR. These cells are perfectly suited for investigating androgen receptor signaling in sertoli cell function in order to determine both molecular and cellular mechanisms.
Procedure Overview
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Propagation	Use of PriCoat? T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. ST38c cells must be grown in Prigrow III (TM003) base medium supplemented with a final concentration of 5% fetal bovine serum (TM999) and a final concentration of 1% Penicillin/Streptomycin (G255). Atmosphere: air: 95%, CO?: 5%; Temperature: 37.0°C.
Quality Control	1. Androgen receptor mRNA was confirmed via RT-PCR while western blot confirmed AR protein expression. 2. Androgen receptor regulated genes were identified via microarray assays. 3. SV40 Tag expression confirmed through fluorescence microscopy
Disclaimer	1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.