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Immortalized-Rat-Retinal-Müller-Cells-(rMC-1)

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Immortalized-Rat-Retinal-Müller-Cells-(rMC-1)

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Immortalized-Rat-Retinal-Müller-Cells-(rMC-1)

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	Print Version
BioSafety Level	II
Organism	Sprague-Dawley Rat
Source Organ	Retina
Growth Properties	Adherent
Morphology	Large, flat cells
Population Doubling	Doubling time of 48 to 52 hours
Recommended Seeding Density	Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Markers	GFAP, CRALBP
Applications	For Research Use Only
Immortalization Method	Serial passaging and Calcium Phosphate mediated transfection with simian virus 40 (SV40) DNA
Description	Müller cells are glial cells in the vertebrate retina responsible for the homeostatic and metabolic support of retinal neurons. Under pathological conditions, a subset of Müller cells may differentiate to neural progenitor/stem cells which regenerate lost photoreceptors and neurons. The rMC-1 cell line is derived from stable transformation of SV40 antigen into primary rat retinal Müller cells. The rMC-1 cells resembles its primary counterpart and expresses reactive gliosis marker GFAP and Müller cell-specific marker CRALBP. This cell line enables mammalian retinal function research, as well as facilitates gene expression studies in Müller cells-neuron and/or Müller cells-endothelial cell interactions.
Procedure Overview
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Propagation	Use of PriCoat? T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The base medium for this cell line is Prigrow III medium available from ABM (TM003). To make the completed growth medium, add the following components to the base medium: 10% fetal bovine serum (TM999) and Penicillin/Streptomycin (G255). Atmosphere: air: 95%, CO?: 5%; Temperature: 37.0°C.
Preservation	1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO. 2. Storage Temperature: Liquid nitrogen vapour phase.
Quality Control	1) Immunocytochemistry and immunoblotting were used to detect the presence of glial fibrillary acidic protein (GFAP) and cellular retinaldehyde-binding protein (CRALBP); 2) Immunocytochemistry was used to confirm the nuclear expression of SV40-LTA in the immortalized cells.
Disclaimer	1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.