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Immortalized-Human-Trophoblast-Cells---hTERT-(Sw.71)

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Immortalized-Human-Trophoblast-Cells---hTERT-(Sw.71)

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Immortalized-Human-Trophoblast-Cells---hTERT-(Sw.71)

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	Print Version
BioSafety Level	II
Organism	Homo sapiens
Source Organ	Placenta
Donor Age	7-week old
Growth Properties	Adherent
Morphology	Polygonal
Population Doubling	24-48 hours
Recommended Seeding Density	Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Markers	Cytokeratin 7 and vimentin
Applications	For Research Use Only
Immortalization Method	Serial passaging and transduction with retroviruses expressing hTERT from the supernatant of pA317-hTERT cell line
Description	The Immortalized Human Trophoblast Cells - hTERT (Sw.71) were derived from 7-week first trimester placenta isolated from normal pregnancy and were virally infected with hTERT. The immortalized cells retained the same morphology as their primary counterparts. It has the abilities to spontaneously fuse as occasional multi-nucleated cells clusters may form, and can secrete cytokines (IL-1β, IL-6, IL-8, Monocyte Chemotactic Protein-1 (MCP-1), GRO (CXCR2), GRO-α (CXCL1), Intercellular Adhesion Molecule-1 (ICAM-1), Osteoprotegerin (OPG), Tissue Inhibitor of Metalloproteinase-1 (TIMP-1), TIMP-2 and VEGF). Cytokeratin 7 and vimentin markers are expressed. The population of cells is determined to be free of immune cells due to the absence of CD45 and CD68, and free of fibroblast cells due to the lack of fibroblast specific antigen. The Sw.71 cells are a valuable tool for trophoblast research.
Procedure Overview
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Propagation	Use of PriCoat? T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The base medium for this cell line is Prigrow IV medium available from ABM (TM004). To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999) to final concentration of 10%, 10mM HEPES, 0.1 mM MEM non-essential amino acids, 1 mM sodium pyruvate, and Penicillin/Streptomycin (G255). Atmosphere: air: 95%, CO?: 5%; Temperature: 37.0°C.
Quality Control	1) hTERT activity was analyzed via TRAPeze ELISA Detection Kit; 2) Human Cytokine Array 3.1 and III was used to determine cytokine profile; 3) Immunocytochemistry was utilized for determining the presence of cell markers.
Disclaimer	1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.