| Overview |
Print Version
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| Product Name |
Anti-HAI-2 (HGFA (Hepatocyte Growth Factor Activator) Inhibitor-2, Placental Bikunin, SPINT2), Cytoplasmic domain Antibody |
| Description |
The antibody is a rabbit polyclonal antibody made to the cytoplasmic domain of human HAI-2. The antibody is made to a synthetic peptide based on the human sequence, and will recognize HAI-2 and not HAI-1. The antibody has been peptide-affinity purified, concentrated to 1 mg/ml, with the addition of 0.05% Sodium Azide as preservative, and 50% glycerol as cryoprotectant.
HAI-2 is an endogenous inhibitor of the kunitz-type serine proteinase HGF-activator (HGFA). HGF was first described as a hepatocyte-specific mitogen and survival factor, and has since been shown to exert a variety of actions on many cell types by binding to its MET receptor. HGF is activated by cleavage of the single-chain form to form a two-chain version by HGFA. HAI-2 also inhibits trypsin, and MTSP-1 (Matriptase-1), a serine proteinase that also activates HGF, and HGF-2 has been shown to inhibit hepsin with nanamolar kIs. HAI-2 was first known as bikunin, and SPINT2 (Serine Proteinase Inhibitor-2), a proteinase inhibitor found in amniotic fluid. HAI-2 message predicts a protein of 252 amino acids, with a predicted mass of 28.23 kDa and a pI of 9.54. A protein termed HAI-2 related small protein is a 106 amino acid protein with little homology to HAI-2, and the HAI-2 antibodies do not recognize the HAI-2 RSP. The HAI proteins are glycosylated, and may have other post-translational modifications, thus the apparent molecular weight is 22-32 Kda kDa for the full-length HAI-2. The chondroitin sulfate groups attached to HAI-2 are thought to be important for its function. The HAI-2 antibodies do not recognize HAI-1. Mice treated with HAI-2 in an ovarian cancer model had smaller tumors, and HAI-2 is reported to be elevated in macrophages during ovarian cancer, and this is considered a favorable indicator of survival. HAI-2 is secreted and membrane-inserted via a type-I transmembrane domain. The carboxyterminal end is a cytoplasmic domain, thought to interact with signaling and cytoskeletal machinery. The extracellular domains include two kringle domains (KD1 and KD2), and KD2 is thought to be inactive or with much lower activity against HGF. In mice the dominant HAI-2 lacks the first kringle domain, but the two-domain form dominates in humans, although in human testis a shorter HAI-2 has been reported, using a second transcription site. HAI-2 is shed from the cell surface by cleavage extracellular to the transmembrane domain. A recommended starting concentration for Western blots is 1:1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species.
FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS.
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| Subtype |
polyclonal |
| Application |
WB |
| Raised In |
Rabbit |
| Specificity |
This antibody is specific for HGFA Inhibitor 2. It will not recognize HGFA Inhibitor 1 and HAI-2 RSP (related small protein).
|
| Immunogen |
Synthetic peptide of Human HGFA Inhibitor 2. |
| Relevance |
HGFA Inhibitor 2 is an endogenous inhibitor of the kunitz-type serine proteinase HGF-activator (HGFA). HGF was first described as a hepatocyte-specific mitogen and survival factor, and has since been shown to exert a variety of actions on many cell types by binding to its MET receptor. HGF is activated by cleavage of the single-chain form to form a two-chain version by HGFA. HGFA Inhibitor 2 also inhibits trypsin, and MTSP-1 (Matriptase-1), a serine proteinase that also activates HGF, and HGF-2 has been shown to inhibit hepsin with nanamolar kIs. HGFA Inhibitor 2 was first known as bikunin, and SPINT2 (Serine Proteinase Inhibitor-2), a proteinase inhibitor found in amniotic fluid. |
| Guarantee |
abm guarantees that all our Anti-HAI-2 (HGFA (Hepatocyte Growth Factor Activator) Inhibitor-2, Placental Bikunin, SPINT2), Cytoplasmic domain Antibody will perform as described on this product webpage, if this is not the case we will provide you with a one-time replacement at no extra cost. Documentation and explanation of experiment conducted will be required when submitting a claim for replacement. |
| Properties |
|
| Formulation |
Liquid |
| Quantity |
100μg |
| Clone |
Polyclonal |
| Storage |
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt. |
| Storage Buffer |
PBS, pH 7.4 with 0.05% sodium azide. |
| Concentration |
1μg/ul |
| Purity |
Immunogen Affinity purified |
| Isotype |
IgG
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| CellularLocalization |
Cell Membrane; single-pass type I membrane protein and Secreted.
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| Species Reactivity |
human |
| Anti-HAI-2 (HGFA (Hepatocyte Growth Factor Activator) Inhibitor-2, Placental Bikunin, SPINT2), Cytoplasmic domain Antibody Images |
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| Specific References |
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Y051605 has not yet been referenced specifically in any publications.
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