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EPHA7 Stable Ba/F3 Cell Line

品牌：Abmgood

貨號：T3069

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EPHA7 Stable Ba/F3 Cell Line

商品詳情參考文獻相關資料

	Print Version
BioSafety Level	II
Organism	Mus musculus
SourceOrgan	Bone Marrow
Growth Properties	Suspension
Morphology	Round
Description	Fusing a kinase domain to a homodimerization domain such as the PNT domain of ETV6 can result in activated kinase. The EPHA7 Stable Ba/F3 Cell Line expresses activated EPHA7 tyrosine kinase that is linked to a GFP reporter. Ba/F3 cells expressing activated tyrosine kinase become completely dependent on the kinase activity for survival and as a result, the cells can be propagated even in the absence of IL-3. EPHA7 belongs to the EPH family of receptor tyrosine kinases consisting of proteins which bind to ephrin. These proteins are expressed in nearly all tissues during embryo development, and they are involved in a variety of developmental processes which include formation of the nervous system, cardiovascular system, and axon guidance. More recently, they have been implicated in ***** biological functions. Increased expression of EPHA7 is associated with multiple forms of carcinoma.
Procedure Overview
Recommended Seeding Density	Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Applications	For Research Use Only
Over Expression	EPHA7 tyrosine kinase (GFP-ETV6-EPHA7) via retroviral transduction
Propagation	The base medium for this cell line is Prigrow II medium available from ABM (TM002). To make the completed growth medium, add the following components to the base medium: 10% fetal bovine serum (TM999), 1% Penicillin/Streptomycin (G255), and 1.2 μg/ml puromycin. Atmosphere: air: 95%, CO?: 5%; Temperature: 37.0°C.
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Quality Control	WB
Disclaimer	1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers. All sales are final. 2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.