五月丁香婷婷综合网,久久久久久久久久久免费,五月色伊人网

您好，歡迎來到拜力生物網(wǎng)站！請登錄注冊

我的訂單 | 聯(lián)系我們 | 在線客服QQ：3012681483 | 客服電話：400-968-7988 | 工作時間：08:30-17:30

主營產(chǎn)品： ELISA試劑盒、抗體、補體血清、兔補體、干擾素、小鼠測溫儀、進口試劑、Quidel代理、Swant代理、PBL Assay Science代理

網(wǎng)站首頁產(chǎn)品中心代理品牌新聞資訊資源中心關(guān)于我們

所在位置：首頁 > 產(chǎn)品中心 > abmgood > abmgood產(chǎn)品

訂購信息

上海拜力生物科技公司

Tel:400-968-7988 021-33779008

TrkA Stable T17 Cell Line

品牌：Abmgood

貨號：T3158

規(guī)格：

貨期：

應(yīng)用：

咨詢客服

立即購買加入購物車

TrkA Stable T17 Cell Line

商品詳情參考文獻相關(guān)資料

	Print Version
BioSafety Level	II
Organism	Mouse
SourceOrgan	Brain
Growth Properties	Adherent
Morphology	Polygonal
Description	TrkA Stable T17 Cell Line expresses rat TrkA (Tropomyosin receptor kinase A) after TrkA plasmid was successfully transfected into SN56 cells and were selected in the presence of G418. TrkA is the receptor for neurotrophins, such as nerve growth factor (NGF), which plays essential role in the nervous system – in particular apoptotic cell death prevention, and/or the promotion of cellular differentiation and axon elongation in neurons. Researchers may use the TrkA Stable T17 Cell Line to screen for NGF-like compounds that would act as TrkA agonists to study the mechanisms of the signaling pathways and affects NGF. The lack of TrkA has been associated with the neurodegenerative processes and thus the cell line is a valuable tool to conduct related studies. abm has available the TrkB Stable T48 Cell Line (T3159), which can be used to screen BDNF-like (brain-derived neurotrophic factor –like) compounds as well.
Procedure Overview
Recommended Seeding Density	Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Applications	For Research Use Only
Over Expression	Rat TrkA receptor via transfection and G418 antibiotic selection
Propagation	Use of PriCoat? T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The base medium for this cell line is Prigrow III medium available from ABM (TM003). To make the completed growth medium, add the following components to the base medium to final concentration of 10% fetal bovine serum (TM999), 1 mM Sodium Pyruvate Solution(TM057), 1% Penicillin/Streptomycin (G255), and 300 mg/ml G418 (G271). Atmosphere: air: 95%, CO?: 5%; Temperature: 37.0°C.
Pictures
Quality Control	Selection with 300 mg/ml G418
References	Jang, S., Okada, M., Sayeed, I., Xiao, G., Stein, D., Jin, P., & Ye, K. (2007). Gambogic amide, a selective agonist for TrkA receptor that possesses robust neurotrophic activity, prevents neuronal cell death. Proceedings of the National Academy of Sciences, 104(41), 16329-16334. doi:10.1073/pnas.0706662104
Disclaimer	1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers. All sales are final. 2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.