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Luc-Akt-PH/YFP Stable MCF-7/B2 Stable Cell Line

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Luc-Akt-PH/YFP Stable MCF-7/B2 Stable Cell Line

品牌：Abmgood

貨號：T3161

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Luc-Akt-PH/YFP Stable MCF-7/B2 Stable Cell Line

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	Print Version
BioSafety Level	II
Organism	Homo sapiens
SourceOrgan	Mammary Tissue
Growth Properties	Adherent
Morphology	Polygonal
Description	Luc-Akt-PH/YFP Stable MCF-7/B2 Stable Cell Line express the pleckstrin homology (PH) domain of Akt fused to Renilla luciferase with a YFP-Mem, allowing the ability to monitor the PIP₃ production in real time through the established BRET biosensor pair. In which the Luc-Akt-PH will be recruited to the plasma membrane due to PIP₃ production which then results in an energy transfer between the luciferase and YFP. The cells are suitable for the screening and study of molecules on the PI3K/Akt pathway for diabetes and cancer-related research. This cell line is the can be used with the Luc-Akt-PH Stable MCF7 Cell Line (available at abm, Cat. T3160) for experimental studies.
Procedure Overview
Recommended Seeding Density	Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Applications	For Research Use Only
Donor Age	69-year Old
Donor Gender	Female
Donor Ethnicity	Caucasian
Over Expression	Luc-Akt-PH through transfection of Luc-Akt-PH vector and selected with 1 mg/ml geneticin to derive stable cline which was then transfected with the pYFP-Mem vector and underwent selection with 1 mg/ml geneticin and 200 μg/ml hygromycin B
Propagation	Use of PriCoat? T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The base medium for this cell line is Prigrow III medium available from abm (TM003). To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999) to final concentration of 10%, 1 mg/ml geneticin (G271), 200 μg/ml hygromycin B (G265), and Penicillin/Streptomycin (G255). Atmosphere: air: 95%, CO?: 5%; Temperature: 37.0°C.
Quality Control	1) Selection preformed with 1 mg/ml geneticin & 200 μg/ml hygromycin B; 2) BRET assay utilized
Disclaimer	1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers. All sales are final. 2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.