|
Print Version |
| BioSafety Level |
II |
| Organism |
Homo sapiens |
| Source Organ |
Umbilical cord blood |
| Donor Gender |
Female |
| Growth Properties |
Adherent |
| Morphology |
Fibroblast-like |
| Passage Number |
19 |
| Population Doubling |
Population doubling time of 23.8 ± 1.9 h |
| Recommended Seeding Density |
Subculture ratio of 1:3 to 1:5 |
| Markers |
CD90, CD34, CD44, CD45, CD166, CD117,CD73, CD105, CK18 and CK19 |
| Applications |
For Research Use Only |
| Immortalization Method |
Serial passaging and transfection with Human Telomerase Reverse Transcriptase (hTERT) expression vector pGRN145 |
| Cell Type Characterization |
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| Description |
Owing to its multiple differentiation abilities, the Mesenchymal stromal cells (MSCs) holds the promise in tissue engineering and regenerative medicine, and displays important clinical potential for cell therapy. The Immortalized Human Cord Blood Mesenchymal Stromal Cells with RFP (cbMSC-hTERT-RFP) retains MSC specific surface markers (CD29, CD73, CD105, CD44 and HLA-ABC) as well as its differentiation capacities into cells of the mesodermal lineage, such as osteoblasts and adipocytes. Karyotype analysis and CGH analysis show no chromosomal abnormality nor genomic deletion or amplifications. In addition, transplantation of cbMSC-hTERT-RFP into the rat model with TBI demonstrates that these cells are readily attracted to the injury area and thus provides a valuable cell-based tool forin vivostem cell research and translational study. |
| Procedure Overview |
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| Propagation |
Use of PriCoat? T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The base medium for this cell line is Prigrow III medium available from ABM (TM003). To make the complete growth medium, add the following components to the base medium: 20% fetal bovine serum (TM999), 4 ng/ml bFGF (Cat. Z101455) and Penicillin/streptomycin. It is recommended to renew medium every 2 to 3 days. Atmosphere: air: 95%, CO2: 5%; Temperature: 37.0°C. |
| Preservation |
1. Freeze Medium: Complete growth medium with 20% FBS and 7% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase. |
| Quality Control |
1) RT-PCR was used to confirm the expression of the hTERT transgene in the immortalized cells; 2) TRAP assay was performed to determine the telomerase activity; 3) Flow cytometry was used to detect surface markers such as CD29, CD73, CD105, CD44 and HLA-ABC; 4) G-banding was used for karyotype analysis; 5) Tumuorigenicity assay was performed to evaluate the cells’ tumorigenic potential. |
| Disclaimer |
1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final.
2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping).
3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information.
4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate. |
