| Overview |
Print Version
|
| Product Name |
Anti-mouse Nerve Growth Factor Antibody |
| Description |
The neurotrophins ("neuro" means nerve and "trophe" means nutrient) are a
family of soluble, basic growth factors which regulate neuronal development,
maintenance, survival and death in the CNS and the PNS.1 NGF, the first
member of the family to be discovered, was originally purified as a factor able
to support survival of sympathetic and sensory spinal neurons in culture.2
It is synthesized and secreted by sympathetic and sensory target organs and
provides trophic support to neurons as they reach their final target.3
Neurotrophin secretion also increases in the nervous system following injury.
Schwann cells, fibroblasts, and activated mast cells normally synthesize NGF
constitutively, however direct trauma and induced cytokines combine to
increase neurotrophin production in these cells after injury.4
NGF is purified in three forms: the 7S, 2.5S and b. The 7S, 130 kDa, form
occurs naturally in mouse submaxillary glands, and is a multimeric protein
composed of two a, one b and two g subunits. The name is
derived from its sedimentation co-efficient, 7S. The biologically active subunit
is the b, which is a 26 kDa dimer composed of two identical 120 amino acid
chains held together by hydrophobic interactions.5 The 2.5S form is 9 amino
acids shorter than the b form, because of proteolysis that occurs during the
purification process.6 The structural hallmark of all the neurotrophins is the
characteristic arrangement of the disulfide bridges known as the cysteine knot,
which has been found in other growth factors such as PDGF.7 There is a 95.8% homology
between the rat and mouse forms, and a 85% homology between the human and mouse.
NGF has been shown to regulate neuronal survival, development function and plasticity.8
Recently, involvement of NGF in processes not involving neuronal cells
has been shown, such as asthma,9 psoriasis10 and wound healing.11
The biological effects of NGF are mediated by two receptors:
TrkA, which is specific for NGF, and p75, which binds all the neurotrophins.12
|
| Subtype |
polyclonal |
| Application |
WB, IHC |
| Raised In |
Rabbit |
| Specificity |
BDNF, NT-3, and NT-4/5: None. |
| Immunogen |
Epitope: Highly purified 2.5 S mouse NGF (Accession P01139). |
| Guarantee |
abm guarantees that all our Anti-mouse Nerve Growth Factor Antibody will perform as described on this product webpage, if this is not the case we will provide you with a one-time replacement at no extra cost. Documentation and explanation of experiment conducted will be required when submitting a claim for replacement. |
| Properties |
|
| Formulation |
Liquid |
| Quantity |
50μl |
| Clone |
Polyclonal |
| Storage |
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt. |
| Storage Buffer |
PBS, pH 7.4 with 0.05% sodium azide. |
| Concentration |
1μg/ul |
| Purity |
Affinity purified on immobilized antigen. |
| Isotype |
IgG |
| Species Reactivity |
human, mouse, rat |
| Anti-mouse Nerve Growth Factor Antibody Images |
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| Specific References |
|
Y051703 has been referenced in 1 publication.
-
1. Roux, P. et al. (2002) Prog. Neurobiol. 67, 203.
2. Levi-Montalcini, R. (1966) Harvey Lect. 60, 217.
3. Farinas, I. et al. (1998) Neuron 21, 325.
4. Levi-Montalcini, R.et al. (1996) Trends Neurosci. 19, 514.
5. Bradshaw, R.A. (1978) Ann. Rev. Biochem 47, 191.
6. Bocchini, V. and Angeletti, P.U. (1969) Proc. Natl. Acad. Sci. USA 64, 787.
7. McDonald, N.Q.et al. (1991) Nature 354, 411.
8. Huang, E.J. and Reichardt, L.F. (2001) Annu. Rev. Neurosci. 24, 677.
9. Freund V. and Frossard, N. (1994) Prog. Brain Res. 146, 335.
10. Raychaudhuri, S.P. and Raychaudhuri, S.K. (2004) Prog. Brain Res. 146, 433.
11. Kawamoto, K. and Matsuda, H. (2004) Prog. Brain. Res. 146, 369.
12. Teng, K.K. and Hempstead, B.L. (2004) Cell Mol. Life Sci. 61, 35.
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