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Print Version |
| BioSafety Level |
II |
| Organism |
Day 14 pregnant Sprague-Dawley rat |
| Source Organ |
Corpora lutea |
| Growth Properties |
Adherent |
| Morphology |
Small and compact with low cytoplasm-to-nucleus ratio at permissive temperature; differentiated, flat monolayer appearance with increased cytoplasm-to-nucleus ratio at non-permissive temperature. |
| Recommended Seeding Density |
Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions. |
| Markers |
IL-6, ER-β, GR, 20α-HSD, JAK2 |
| Applications |
For Research Use Only |
| Immortalization Method |
Serial passaging and infection by SV-40 tsA209 Temperature Sensitive Mutant virus |
| Description |
The corpus luteum develops from an ovarian follicle during the luteal phase of the menstrual cycle and is responsible for the decidualization of the endometrium. The Immortalized Rat Luteal Cells (GG-CL) is conditionally immortalized through the temperature-sensitive simian virus (SV40 tsA209) and has retained many primary luteal cell markers such as estrogen receptor-β, glucocorticoid receptor (GR), interleukin-6 (IL-6), orphan nuclear receptor nur 77 and 20α-hydroxysteroid dehydrogenase (20α-HSD). In addition, GG-CL responds to stimulation by PRL, estradiol, and cAMP, making the cells a useful model for studies involving gene expression and regulation of luteal cells. |
| Procedure Overview |
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| Propagation |
Use of PriCoat? T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The base medium for this cell line is Prigrow II medium available from ABM (TM002). To make the complete growth medium, add the following components to the base medium: 10% fetal bovine serum (TM999) and Penicillin/streptomycin. Atmosphere: air: 95%, CO2: 5%; Temperature: 33.0°C. The functional expression of the SV40 antigen is induced by culturing the cells in vitro at a temperature permissive (33°C). At a non-permissive temperature (37°C-39°C), the cells cease to proliferate and revert to differentiated phenotype. |
| Preservation |
1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase. |
| Quality Control |
1) Northern analysis and RT-PCR were used to confirm the expression of IL-6, glucocorticoid receptor (GR), estrogen receptor-β (ER-β) and orphan nuclear receptor nur 77. 2) Cells were subjected to treatments of exogenous prolactin, estradiol, and cAMP to examine effects. |
| Disclaimer |
1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final.
2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping).
3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information.
4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate. |
