Direct non-viral plasmid transfection for immediate expression
Package into Lentiviral particles for high efficiency transduction and stably integrated expression
miRNA ID
MIMAT0000747
miRNA Name
mmu-miR-382-5p
Description
This miRNA lentiviral vector is part of abm’s Lentivirus Expression System and can be packaged into virus using an appropriate packaging mix to over-express your miRNA of interest in a wide range of host cells or animal models. This construct contains a GFP reporter for monitoring transfection efficiency.
Accession No.
MIMAT0000747
Species
Mouse
Insert Type
Mature miRNA
Vector Name
pLenti-III-Cumate-mico-GFP
Vector Map
Viral Titer
N/A
Tools and Links
User Manual
Background Reading
Reference
Markusic D, Oude-Elferink R, Das AT, Berkhout B, Seppen J. Comparison of single regulated lentiviral vectors with rtTA expression driven by an autoregulatory loop or a constitutive promoter. Nucleic Acids Res 2005;33:e63
Disclaimer
abm guarantees that the correct construct is provided and GFP expression is displayed upon successful transduction. If this is not the case, we will provide a one-time replacement. Customers must provide adequate data to show >80% transfection efficiency with a positive control. The replacement will not be covered by the same guarantee.
Please note that due to the large number of variables applicable, any further expression analysis is not covered by the guarantee, as such analysis is dependent on the end user's experimental conditions