This broad spectrum Protease and Phosphatase Inhibitor cocktail has been specifically designed to provide complete protection of valuable proteins of interest from degradation during extraction and purification.
Features and Benefits: Complete protection from proteases and phosphatases in one single cocktail.
- All our cocktails contain AEBSF, the preferred serine protease inhibitor, to provide superior protection with unmatched stability, convenience and reliability.
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- The cocktail formulations are available with or without EDTA which may interfere with protein stability or certain downstream applications.
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- Our cocktails are lyophilized solids which dissolve instantaneously making them readily available for their instantaneous inhibitory action and are easier to use than tablets that require splitting, vortexing and time consuming dissolution.
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- Our lyophilized solids are guaranteed for stability, reproducibility and have a shelf life of over a year. Most other commercially available cocktails made and sold in aqueous solutions offer very low guarantee of stability after 1 month of being manufactured (Lit ref: Bestatin, Leupeptin solution in water are stable for 1 month at -20oC).
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- Economical: More cost effective than other commercially available formulations.
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- Reliable: Count on our expertise in the production of high quality cocktails that have been successfully used for 20 + years by thousands of researchers worldwide.
Product Description: White Lyophilized Solid. The cocktail contains 16 inhibitors targeting against the major classes of proteases and phosphatases as outlined in the following table
Products | Target Proteases | Products | Target Phosphatases |
AEBSF, HCI | Serine Proteases | Imidazole | Alkaline Phosphatases |
Aprotinin | Broad Spectrum, Serine Proteases | Sodium Orthovanadate | Protein Tyrosine Phosphatase, Alkaline Phosphatase |
Bestatin | Aminopeptidase B and Leucine Aminopeptidase | Sodium Molybdate | Acid and Phosphoprotein Phosphatases |
E-64 | Cysteine Proteases | Na F | Acid Phosphatases |
Leupeptin | Cysteine Proteases and Trypsin-like Proteases | Sodium Pyrophosphate | PP1 and PP2A |
Pepstatin A | Aspartic Proteases | Glycerophosphate | Ser/Thr Phosphatases |
Phosphoramidon | Metalloproteases Metalloendopeptidases | Na Tartrate Dihydrate | Acid Phosphatases |
Antipain | Papain, Cathepsin A/B | Bromotetramisole oxalate | ? |
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Recommended Usage: This cocktail has been optimized for effectiveness, convenience, stability, reproducibility and has been tested for general use. It inhibits activity in extracts from almost any tissue or cell types including animals, plants, yeasts, bacteria and fungi.
Reconstitute each vial with 1 ml of water to obtain a concentrated stock solution. Dilute this conc stock solution to 1X before use. Recommended dilution is 1:100 (based on the application). One vial is recommended for 10g-20g of tissue or cell extract.
Performance Activity Assay:?Comparison of our Turbocharged Protease and Phosphatase Inhibitor Cocktails with Competitor’s commercial tablets.?
Figure 1:?Using a general protease activity assay and pancreas extract, our Turbocharged Cocktails were tested against Competitor’s commercially available tablets under the same conditions. The results were based on protease activity in the absence of any inhibitor cocktails (Test 1); compared to protease activity in the presence of Competitors Protease Inhibitor Tablets (Test 2); Turbocharged Protease Inhibitor Cocktail (T-2492/Test 3); Turbocharged Protease + Phosphatase Inhibitor Cocktail (T-2495/Test 4); and Blank
Figure 2:?Using a general phosphatase activity assay the alkaline phosphatase activity was determined with no inhibitor cocktail added (Test 1); compared to Phosphatase activity in the presence of Turbocharged Phosphatase Inhibitor Cocktail (T-2494/Test 2); Turbocharged Protease + Phosphatase Inhibitor Cocktail (T-2495/Test 3); and Blank?
The results indicate that our Turbocharged Cocktails showed better performance ( > 97% inhibition) than the Competitors product with our Turbocharged Protease + Phosphatase inhibitor Cocktails being the only one of its kind available in the market today inhibiting both Proteases + Phosphatases available as a lyophilized solid in a single vial.
Additional Information
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Product # | T-2495 |
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Appearance | Lyophilized Solid. Hygroscopic |
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Purity | Suitable for use as Protease and Phosphatase Inhibitors |
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Solubility | Soluble in water, aqueous buffers or add directly to extraction media |
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Storage Temp | -20oC |
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Additional Information
