5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside is most commonly known as?X-Gal. X-gal is a histochemical substrate for β-galactosidase which cleaves X-Gal to yield an insoluble blue precipitate.
X-Gal is a mainstay in molecular biology cloning applications in which it is used to detect the activity of β-galactosidase. X-Gal is used to detect the insertion of foreign DNA into the lacZ region of a plasmid DNA. Insertion of DNA into the lacZ region results in the loss of β-galactosidase activity.
X-gal is used to indicate whether a cell expresses the β-glactosidase enzyme, which is encoded by the lacZ gene, in a technique called blue white screening. Bacterial cells that retain active β-galactosidase will result in characteristic blue colonies. Successful disruption of the?lacZα?gene disrupts the α-complementation of the β-galactosidase gene and the precipitate does not form, resulting in white colonies.
?
BIOLEAF熱搜 BIOLEAF試劑盒 BIOLEAF ELISA BIOLEAF試劑 BIOLEAF品牌 BIOLEAF抗體 BIOLEAF耗材 BIOLEAF小儀器
sitemap 細(xì)胞庫(kù)查詢 危險(xiǎn)品圖標(biāo) 本公司網(wǎng)站所展示銷(xiāo)售的產(chǎn)品僅供科研！
滬公網(wǎng)安備 31011202007338號(hào)
