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Details

Host:?Rabbit

Isotype:?IgG

Class:?Polyclonal

Immunogen:?Synthetic peptide corresponding to Human c-Myc protein

Applications:

Red-colored affinity matrix consists of affinity purified anti-c-Myc rabbit polyclonal antibody covalently attached to red beaded agarose (Separopore?). The c-Myc tag corresponds to amino acid residues 410-419 (EQKLISEEDL) of the human c-Myc protein. Anti-cMyc Red-Separopore? 4B-CL is convenient for quantitative capturing of antigen/antibody complexes in immunoprecipitation, greatly reduces the risk of pellet loss. Comparable performance to non-colored affinity gels, but allows the user to easily differentiate pellet from supernatant. This results in greater consistency and more accurate data due to less sample losses. Anti-cMyc Red-Separopore? 4B-CL is reliable tool?for affinity capture (immunoprecipitation) of N- or C-terminal c-Myc-tagged fusion proteins from?E. coli?or mammalian cells.

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Red Separopore ??Technical Specifications:

• Matrix: Separopore? 4B-CL (crosslinked agarose beads, 4%)
• Concentration:?25 mg Red Separopore ? per mL of buffer
• Particle size range: 52 – 165 μm
• Ligand coupling method: CNBr activation?
• Binding capacity: approximately 50 μg/ml drained gel
• Exclusion limit: approximately 2 x 10⁷
• pH stability: 3 – 9 (for use), 2 – 10 (for cleaning)?
• Supplied as 50% suspension in PBS, containing 50% glycerol.?

Additional Information

Additional Information