Cycloheximide is an antibiotic active against many yeasts and fungi. Inhibits protein synthesis in eukaryotes (but not in prokaryotes) by interfering with the translocation step. It inhibits chain initiation as well as chain elongation by acting on the 60S subunit of the eukaryote ribosome, interacting directly with enzyme translocase. Cycloheximide is used as an inhibitor to study cell-free protein biosynthesis in eukaryotes and also used to block ribosome-dependent in vivo polypeptide synthesis. It induces apoptosis in a variety of cells, but can also delay or inhibit apoptosis by other agents.
Cycloheximide’s?longstanding application as a gene selection antibiotic?has a powerful new role in Genome editing utilizing the CRISPR/Cas9 system.?A Candida albicans CRISPR system permits genetic engineering of essential genes and gene families.
Cycloheximide is in stock. Call for bulk quotations, typical lead for subdivision, packing and shipping larger quantities is 7-14 days.Research size order 100mg 250mg lead is shorter.International Shipping of this material can be subject to an additional hazardous shipping fee.
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