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Ready-to-use fluorometric substrate for caspase-1,-4,-5 and related caspases that recognize the amino acid sequence WEHD. Caspase-5 and related caspase activity can be quantified by fluorescent detection of free AFC after cleavage from the peptide substrate WEHD-AFC at Ex. = 400 nm and Em. = 505 nm, using a fluorometer or multi-well fluorescence plate reader. Alternatively, a shift in fluorescence from blue to green upon cleavage can be visualized using a hand-held long-UV lamp. The ready-to-use caspase substrate provides an economic alternative for researchers who perform large amount of caspase assays. Cell Lysis Buffer (Cat. #1067-100, -400) and 2X Reaction Buffer (Cat. #1068-20, -80) for caspase assays are also available separately.

Additional Information

Synonyms	Ac-WEHD-AFC, Caspase-5 Substrate, Fluorogenic
Product #	C-2245
Chemical Name	Ac-WEHD-AFC, Caspase-5 Substrate, Fluorogenic
Formula	C38H37F3N8O11
MW	838.8
Appearance	Liquid
Purity	99% by HPLC
Storage Temp	-20°C

Additional Information

MDL Number	MFCD01862607
ChemACX	X1340880-7
InChI	InChI=1S/C38H37F3N8O11/c1-18(50)45-27(10-19-15-43-25-5-3-2-4-22(19)25)36(58)47-26(8-9-31(51)52)34(56)48-28(11-21-16-42-17-44-21)37(59)49-29(14-32(53)54)35(57)46-20-6-7-23-24(38(39,40)41)13-33(55)60-30(23)12-20/h2-7,12-13,15-17,26-29,43H,8-11,14H2,1H3,(H,42,44)(H,45,50)(H,46,57)(H,47,58)(H,48,56)(H,49,59)(H,51,52)(H,53,54)
SMILES	CC(=O)NC(CC1=CNC2=CC=CC=C21)C(=O)NC(CCC(=O)O)C(=O)NC(CC3=CN=CN3)C(=O)NC(CC(=O)O)C(=O)NC4=CC5=C(C=C4)C(=CC(=O)O5)C(F)(F)F
Peptide Sequence	AC-TRP-GLU-HIS-ASP-AFC