Lipodin-Pro? is the ultimate transfection reagent that transports biologically active peptides, proteins and antibodies directly into living cells. Lipodin-Pro? represents an alternative to nucleic acid transfection for functional studies. Lipodin-Pro? is a lipid-based formulation that is added to the protein of interest minutes before cell delivery. Within few hours, protein activity can be observed in living or fixed cells. Regardless of serum addition, Lipodin-Pro? is ideal for monitoring kinetics of biological activities in established cell lines and primary cells, including neurons.
Protein Delivery Applications for Lipodin-Pro? Lipodin-Pro can be used in various functional studies for cell signaling and apoptotic assays, protein-protein interaction, protein localization and compartment shuttling. When the protein is conjugated to a fluorescent dye, the functional assay can be carried out in living cells under multiple treatments with a single sample. Lipodin-Pro successfully delivered numerous proteins in a wide variety of cells: B and R-phycoerythrin, bovine serum albumin, β-galactosidase, human active caspase-3 and various immunoglobulins (unconjugated or labeled with FITC, TRITC, AlexaFluor?488 and AlexaFluor?546). Standard cell lines and primary cells were successfully assayed for Lipodin-Pro reagent.
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