Endotoxin is the lipopolysaccharide (LPS) complex located in the outer membrane of gram-negative bacteria. A single E.coli bacterium contains ~2 million LPS molecules (2 – 20 fg/cell). During experimental procedures, large amount of endotoxins are shed and can easily contaminate labware, buffers and downstream products.
In vitro, endotoxin causes a variety of problems in cell-based research. In vivo, endotoxin may cause various side effects, including inflammatory response, organ failure or septic shock in host organisms. Presence of endotoxin in any of these has a detrimental effect on culturing of cells and can lead to generation of irreproducible or negative experimental data. Therefore, it is critical to remove endotoxin from samples and products. Endotoxin Rapid Removal Agarose can quickly and effectively eliminate endotoxins to < 0.05 EU/ml in solutions containing proteins or pharmacologically important components via the immobilized polymyxin B, which is known for capturing endotoxin and preventing toxic effects.
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