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Calmodulins immobilized via SH (wheat germ) and amino groups (porcine) to epoxy resin allowing multiple orientations of bound calmodulin for efficient capture of calmodulin binding proteins. Wheat germ (Triticum aestivum) calmodulin (CaM) sequence is unique in that it contains cysteine at residue position 26 (Cys26). Wheat germ calmodulin coupled primarily through SH group (Cys26) to epoxy activated resin. Coupled at 5 mg per mL and useful for pull down experiments and proteomic applications requiring a high capacity precipitation reagent. Dense resin can be easily separated from extracts by centrifugation using batch or spin column procedures. Resin can be reused but proteolysis from crude samples will reduce binding capacity on repeat cycles. Loading conditions: 100M Ca2+, with protease inhibitors and appropriate buffers. Elution conditions: Buffers with 1 mM EDTA or EGTA.

Additional Information

Synonyms	CaMTag? HD Capture plus
Product #	C-1441
Chemical Name	CAMTAG? HD CAPTURE PLUS
Storage Temp	+4°C

Additional Information