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Ready-to-use colorimetric substrate for caspase-5 and related caspases that recognize the amino acid sequence WEHD. Caspase-5 and related caspase activity can be quantified by spectrophotometric detection of free pNA (l = 400 nm) after cleaved from the peptide substrate WEHD-pNA, using a spectrophotometer or multi-well plate reader. The ready-to-use caspase substrate provides an economic alternative for researchers who perform large volume of caspase assays.

Additional Information

Synonyms	Ac-WEHD-pNA, Caspase-5 Substrate, Colorimetric
Product #	C-2246
Chemical Name	Ac-WEHD-pNA, Caspase-5 Substrate, Colorimetric
Formula	C34H37N9O11
MW	747.7
Appearance	Liquid
Purity	99% by HPLC
Storage Temp	-20°C
Use	Colorimetric substrate for caspase-5 and related caspases that recognize the amino acid sequence WEHD

Additional Information

MDL Number	MFCD03095617
ChemACX	X1340884-5
InChI	InChI=1S/C34H37N9O11/c1-18(44)38-26(12-19-15-36-24-5-3-2-4-23(19)24)33(51)40-25(10-11-29(45)46)31(49)41-27(13-21-16-35-17-37-21)34(52)42-28(14-30(47)48)32(50)39-20-6-8-22(9-7-20)43(53)54/h2-9,15-17,25-28,36H,10-14H2,1H3,(H,35,37)(H,38,44)(H,39,50)(H,40,51)(H,41,49)(H,42,52)(H,45,46)(H,47,48)
SMILES	CC(=O)NC(CC1=CNC2=CC=CC=C21)C(=O)NC(CCC(=O)O)C(=O)NC(CC3=CN=CN3)C(=O)NC(CC(=O)O)C(=O)NC4=CC=C(C=C4)[N+](=O)[O-]
Peptide Sequence	AC-TRP-GLU-HIS-ASP-PARANITROANILIDE