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Applications:

Protein A binds specifically to F_c?regions of many mammalian immunoglobulins.?Protein A-conjugates are commonly used as affinity adsorbents to purify immunoglobulins (antibodies) and immunoglobulin subtypes from serum, hybridoma ascites fluids, tissue culture supernatants and other biological fluids. Protein A- 4B-CL is used for purification and/or fractionation of IgG subclasses. It is a reliable tool to purify monoclonal and polyclonal IgG from ascites, serum and cell culture supernatants.?This is also commonly used to capture immune complexes generated in immunoprecipitation (IP) experiments.

Technical Specifications

• Ligand:?recombinant Protein A from?E. coli
• Matrix: 4B-CL?(crosslinked agarose beads, 4%)
• Particle size range: 52 – 165 μm
• Matrix activation: Cyanogen bromide
• Molecular weight range: 6 x 10⁴?-? 2 x 10⁷
• Ligand density:? ~3 mg Protein A / ml drained gel
• Binding capacity: ~ 20 mg human IgG / ml drained gel
• pH stability: 3 – 9 (for use), 2 – 10 (for cleaning)
• Storage: 2 – 8 °C.
• Supplied as 50% slurry in PBS with sodium azide

Additional Information

Additional Information