OxiRed, which has been marketed under the name Amplex Red? reagent, is a highly sensitive and stable probe for H2O2, is a fluorogenic (EX/EM = 535/587 nm) and Colorometric (l = 570 nm) substrate for peroxidase. In the presence of horseradish peroxidase (HRP), the OxiRed probe reacts in a 1:1 stoichiometry with H2O2 to produce highly fluorescent resorufin. Because H2O2 is produced in many different enzymatic reactions, the OxiRed probe allows researchers to detect the activity of many different enzymes either colorimetrically or fluorometrically.
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