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Applications:

Provides immobilized?E. coli?proteins on a solid bio-affinity support for the removal of antibodies to?E. coli?proteins from antisera prepared against recombinant proteins expressed in?E. coli. Our immobilized?E. coli?lysate consists of partially purified proteins from?E. coli?strains commonly used for protein expression. The coupling chemistry is leak-resistant and provides a matrix with very low nonspecific binding. A column of immobilized?E. coli?lysate thus functions as a "reverse affinity matrix". The contaminating?E. coli?antibodies are absorbed onto the matrix and the purified antisera pass?through for further use.

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Technical specifications:

• Ligand:?E. coli?proteins?that are extracted from three strains
• Matrix: Separopore?^?4B?(agarose beads, 4 %)
• Particle size range: 52 – 165 μm
• Molecular weight range: 6 x 10⁴?- 2 x 10⁷
• Matrix activation: Cyanogen bromide
• pH stability: 4 – 9
• Storage?temp:?4?°C?in the presence of sodium azide

Additional Information

Additional Information