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Applications:

Endotoxin Affisorbent consists of immobilized polymyxin B on 4B-CL. The polymyxins are a family of antibiotics that contain a cationic cyclopeptide with a fatty acid chain. Polymyxin B neutralizes the biological activity of endotoxins by binding to the lipid A portion of bacterial lipopolysaccharide. The immobilized polymixin B gel is a stable affinity matrix that resists leaching of ligand into the valuable preparation.?Affisorbent Endotoxin Removing Gel can also be used to remove endotoxins from nucleic acid (DNA) samples.Polymyxin B-4B-CL is used in protein chromatography, affinity chromatography and specialty resins. Polymyxin B- 4B-CL has been used to study periodontitis and endodontic infections as well as obesity and type 2 diabetes.?Polymyxin B- 4B-CL?is also used for the removal of?endotoxin levels in protein solutions, cell culture media, ascites.

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Technical Specifications:

• Ligand:?Polymyxin B
• Matrix:?4B-CL (crosslinked agarose beads, 4%)
• Particle size range:?52 – 165 μm
• Matrix activation:?Cyanogen bromide
• Attachment:?Amino group
• Binding capacity:?200 – 500 μg?LPS from?E. coli?serotype 0128:B12
• pH stability:?3 – 10
• Supplied as?slurry in 50% glycerol containing 0.02% sodium azide
• Storage temp: 2 – 8 °C

Additional Information

Additional Information