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Applications:

• Rapid immobilization of antibodies and other proteins to the matrix.
• Immobilization of ligands through primary amines to purify recombinant proteins.
• Immobilization of Protein A or Protein G for monoclonal antibody purification.
• Pre-activated medium for immobilization of ligands containing a primary amino group.
• N-hydroxyl succinimide (NHS) ester forms a stable amide (peptide) bond with the primary amines of ligands.
• The stability of an amide bond at high pH (up to pH 13) allows for a very stringent wash protocol during affinity purification using NHS-activated ligand coupling.
• Coupling can be carried out in neutral pH which makes it ideal for immobilization of antibodies, antigens, and other proteins sensitive to pH extremes.
• Coupling reaction can also be carried out in organic solvents.
• Provides a 10 atom spacer arm upon ligand coupling.
• Coupling completed after 2 – 4 h at room temperature.
• NHS-Activated provides a valuable tool for affinity purification of antibodies, antigens and other biomolecules.

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Technical specifications:

• Group to be coupled: Primary -NH₂
• Matrix: 4B-CL (highly crosslinked agarose beads, 4%)
• Particle size range: 52 – 165 μm
• Molecular weight range: 6 x 10⁴?– 2 x 10⁷
• Chemical linkage of ligand: Amide bond
• Spacer arm: 6-aminocaproic acid, 10 atoms
• Substitution: >18 μmol NHS / ml drained gel
• pH stability: 3 – 13 (ligand dependent)
• Coupling conditions: pH 6 – 9; Temp: 4 – 25 °C
• Storage: 2 – 8 °C
• Flow rate specifications: 70 – 140 cm / h
• Supplied as a pre-swelled gel suspension in 100% isopropanol to protect the active groups

Additional Information

Additional Information