最近最新的日本中文MV_麻豆出品视频在线_超pen个人视频97_国产精品第一国产精品_国产香蕉片_免费看麻豆文化传媒_奶大灬舒服灬太大了一进一出_人妻激情偷乱一区二区三区

HLY78 is an activator of the Wnt pathway by acting in a Wnt ligand–dependent manner, identified by a high-throughput, cell-based screen. HLY78 targets the DIX domain of Axin and potentiates the Axin–LRP6 association, thus promoting LRP6 phosphorylation and Wnt signaling transduction. HLY78 may weaken the autoinhibition of Axin. In addition, HLY78 acts synergistically with Wnt in the embryonic development of zebrafish and increases the expression of the conserved hematopoietic stem cell (HSC) markers, runx1 and cmyb, in zebrafish embryos. HLY78 as a specific Wnt synergistic activator presents another opportunity for potential clinical applications relevant to the Wnt pathway.

?

How to Use:

• In vitro:??HLY78 was used at 10-20 μM final concentration in various in vitro assays.
• In vivo:?Wild-type zebrafish embryos were treated with HLY78 at 80 μM from the three-somite stage until 36 h.p.f. were fixed for in situ hybridization with a mixture of runx1/cmyb probe.

?

Reference:

• 1. ?Wang S, et al. Small-molecule modulation of Wnt signaling via modulating the Axin-LRP5/6 interaction. (2013) Nat Chem Biol. 9(9):579-85.

?

?

Products are for research use only. Not for human use.