Biological Activity:
UM171 is a potent small molecule that stimulates the ex vivo expansion of human hematopoietic stem cells/HSC capable of reconstituting human hematopoiesis for at least 6 months in immunocompromised mice. It attenuates cell differentiation and promotes ex vivo expansion of long-term human HSC (LT-HSC) at 35 nM in 11 days fed-batch cultures. UM171’s mechanism is independent of suppression of the aryl hydrocarbon receptor (AHR) like SR1. The properties of UM171 make it a potential candidate for hematopoietic stem cell transplantation and gene therapy.
How to Use:
In vitro: ?For transplantation experiments, the fed-batch culture system was used. 1x105 CD34+ cord blood (CB) cells/ml were injected into 12 ml or 25 ml bags connected to a syringe loaded pumping system and maintained on an orbital shaker at 37°C and 5% CO2 in air. The pump was set to continuously deliver HSC expansion media supplemented with UM171 (35 nM) at a flow rate of 0.7 μl/min.?
In vivo: NSG mice were injected with CD34+ CB cells that had been originally cultured in UM171.
Reference:
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