Product Information
| Purity: |
Affinity purification |
| Storage: |
Store at -20 or -80°C. Avoid freeze/thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
Catalog No: A70023
Histone H3K9me2 Polyclonal Antibody
Application:
WB (1:500-1:2000), IHC (1:50-1:200), IF (1:50-1:200), IP (1:50-1:200), ChIP (1:50-1:200)
Cross-Reactivity: H, M, R, other (wide range)
Calculated MW: 15kDa
Species: Rabbit
Isotype: IgG
Purity: Affinity purification
Concentration: 1mg/mL
Storage:
Store at -20 or -80°C. Avoid freeze/thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Immunogen Information
Immunogen:
A synthetic methylated peptide corresponding to residues surrounding Lys9 of human histone H3
Gene ID: 8290
Swiss Prot: Q16695
Synonyms:
HIST1H3J; H3/j; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/I; Histone H3/j; Histone H3/k; Histone H3/I; HIST3H3
Background:
H3K9 Methylation is one of the gene-repressive modification markers. Particularly, H3K9me3 is highly correlated with constitutive heterochromatin. H3K9me3 is found in constitutively repressed genes (1). Closely-related H3K9 HMTases include GLP and G9a (2, 3, 4). H3K9 can also be modified by acetylation/deacetylation. H3K9ac is found in actively transcribed promoters (5, 6). To study H3K9 methylation status, check our K3K9 Methylation series.
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Reference:
1. Philip H, et al. Mechanisms of Transcriptional Repression by Histone Lysine Methylation". (April 2009). The International Journal of Developmental Biology (Basel) 10 (1387): 335–354.
2. Vedadi M, et al. A chemical probe selectively inhibits G9a and GLP methyltransferase activity in cells. (2011) Nat Chem Biol. 7(8): 566-74.
3. Kubicek S. et al. Reversal of H3K9me2 by a small-molecule inhibitor for the G9a histone methyltransferase. (2007) Mol. Cell 25, 473–481.
4. Shi Y, et al. Induction of pluripotent stem cells from mouse embryonic fibroblasts by Oct4 and Klf4 with small-molecule compounds. (2008) Cell Stem Cell. 3(5): 568-74
5. Malvaez M, et al. HDAC3-selective inhibitor enhances extinction of cocaine-seeking behavior in a persistent manner. (2013) Proc Natl Acad Sci USA. 110(7): 2647-52.
6. Wells CE, et al. Inhibition of histone deacetylase 3 causes replication stress in cutaneous T cell lymphoma. (2013) PLoS One. 8(7): e68915.
