間充質(zhì)干細胞培養(yǎng)基是專門為人類間充質(zhì)干細胞體外培養(yǎng)所設(shè)計的、*適合其生長的培養(yǎng)基。是經(jīng)滅菌的液體培養(yǎng)基，包含必需和非必需氨基酸、維生素、有機和無機化合物、激素、生長因子、微量礦物質(zhì)和低濃度胎牛血清(5%)。該培養(yǎng)基緩沖體系為重碳酸鹽，在含5%CO2的細胞培養(yǎng)箱中平衡后pH值為7.4。該培養(yǎng)基的配方能夠選擇性的促進正常人類微血管內(nèi)皮細胞體外培養(yǎng)中的增殖和生長，并為其達到*理想營養(yǎng)平衡狀態(tài)提供數(shù)量上和質(zhì)量上的保證。
間充質(zhì)干細胞培養(yǎng)基包含500 ml基礎(chǔ)培養(yǎng)基，25ml胎牛血清(FBS,目錄編號0025)，5ml間充質(zhì)干細胞生長添加物，(MSCGS,目錄編號7552)和5 ml青霉素/鏈霉素溶液(P/S,目錄編號0503)
|
貨號
|
7501
|
|
產(chǎn)地
|
美國
|
|
縮寫
|
MSCM
|
|
規(guī)格
|
500ml
|
|
用途
|
科研
|
|
儲存
|
4度，-20度
|
|
運輸
|
膠冰
|
1.) Reed SA, Johnson SE. (2013) "Expression of scleraxis and tenascin C in equine adipose and umbilical cord blood derived stem cells is dependent upon substrata and FGF supplementation."?Cytotechnology. doi. 10.1007/s10616-012-9533-3.
2.) Johnson SP, Catania JM, Harman RJ, Jensen ED. (2012) "Adipose-derived stem cell collection and characterization in bottlenose dolphins (Tursiops truncatus)."?Stem Cells Dev. 21: 2949-57.?
3.) Ma Z, Gao BZ. (2012) "Quantitatively analyzing the protective effect of mesenchymal stem cells on cardiomyocytes in single-cell biochips."?Biotechnol Lett. 34: 1385-91.?
4.) Ma Z, Liu Q, Liu H, Yang H, Yun JX, Xu M, Eisenberg CA, Borg TK, Markwald R, Gao BZ. (2012) "Cardiogenic Regulation of Stem-Cell Electrical Properties in a Laser-Patterned Biochip."?Cell Mol Bioeng. 5: 327-336.?
5.) Panicker SP, Ganguly T, Consolo M, Price V, Mirmirani P, Honda K, Karnik P. (2012) "Sterol intermediates of cholesterol biosynthesis inhibit hair growth and trigger an innate immune response in cicatricial alopecia." PLoS One. 7: e38449.?
6.) Suh N, Paul S, Lee HJ, Yoon T, Shah N, Son AI, Reddi AH, Medici D, S**** MB. (2012) "Synthetic triterpenoids, CDDO-Imidazolide and CDDO-Ethyl amide, induce chondrogenesis."?Osteoarthritis Cartilage. 20: 446-50.?
7.) Sun HP, Zhang X, Chen XH, Zhang C, Gao L, Feng YM, Peng XG, Gao L. (2012) "Human umbilical cord blood-derived stromal cells are superior to human umbilical cord blood-derived mesenchymal stem cells in inducing myeloid lineage differentiation in vitro."?Stem Cells Dev. 21: 1429-40.
8.) Wang Y, Gao R, Wang PP, Jian J, Jiang XL, Yan C, Lin X, Wu L, Chen GQ, Wu Q. (2012) "The differential effects of aligned electrospun PHBHHx fibers on adipogenic and osteogenic potential of MSCs through the regulation of PPAR? signaling."?Biomaterials. 33: 485-93.?
9.) Choi KS, Ahn SY, Kim TS, Kim J, Kim BG, Han KH, Ban SJ, Kim HS, Choi Y, Lim CJ. (2011) "Characterization and biodistribution of human mesenchymal stem cells transduced with lentiviral-mediated BMP2."?Arch Pharm Res. 34: 599-606.?
10.) Dissanayaka WL, Zhu X, Zhang C, Jin L. (2011) "Characterization of dental pulp stem cells isolated from canine premolars."?J Endod. 37: 1074-80.?
11.) Zhao Z, Chen Z, Zhao X, Pan F, Cai M, Wang T, Zhang H, Lu JR, Lei M. (2011) "Sphingosine-1-phosphate promotes the differentiation of human umbilical cord mesenchymal stem cells into cardiomyocytes under the designated culturing conditions."?J Biomed Sci. 18: 37.
12.) Gong Z, Calkins G, Cheng EC, Krause D, Niklason LE. (2009) "Influence of culture medium on smooth muscle cell differentiation from human bone marrow-derived mesenchymal stem cells."?Tissue Eng Part A. 15: 319-30.
13.) Reed SA, Johnson SE. (2008) "Equine umbilical cord blood contains a population of stem cells that express Oct4 and differentiate into mesodermal and endodermal cell types."?J Cell Physiol. 215: 329-36.?
Question:
What is the glucose concentration of this medium?
Answers:
5.55 mM
