Macrophages are cells differentiated from circulating bone
marrow-derived monocytes. The main function of macrophages is to remove
cellular debris and destroy invading pathogens. Mouse Splenic
Macrophages (MSMa) reside in the spleen and play an critical role in
maintaining blood homeostasis [1]. There are three distinct populations
of splenic macrophages including: red pulp macrophages, marginal zone
macrophages, and marginal zone metallophillic macrophages [1, 2]. MSMa
perform iron processing functions and aid in the capture of microbes and
viruses in the circulatory system [1, 2]. Recent studies have shown
that splenic macrophages contribute to stroke pathology and autoimmune
diseases [3, 4]. MSMa are an excellent model for studying macrophage
functions under normal physiological and pathological conditions.
MSMa from ScienCell Research Laboratories are isolated from *****
C57BL/6 mouse spleen. MSMa are cryopreserved after purification and
delivered frozen. Each vial contains > 1 x 106 cells in 1 ml volume.
MSMa are characterized by immunofluorescence with antibody to F4/80.
MSMa are negative for mycoplasma, bacteria, yeast, and fungi. MSMa are
guaranteed to further culture in the conditions provided by ScienCell
Research Laboratories; however, MSMa are not recommended for expanding or long-term cultures since the cells do not proliferate in regular culture.
