血管內(nèi)皮細(xì)胞有維持血管內(nèi)環(huán)境平衡的功能，血管內(nèi)皮細(xì)胞產(chǎn)生、分泌纖維蛋白凝集和分解系統(tǒng)的活化劑和抑制劑。另外它們調(diào)控血小板的粘附和聚集。內(nèi)皮細(xì)胞同時(shí)釋放分子，調(diào)控細(xì)胞增殖，控制血管壁功能。人臍靜脈內(nèi)皮細(xì)胞是應(yīng)用*普遍的進(jìn)行體外研究內(nèi)皮細(xì)胞的細(xì)胞類型。臍靜脈內(nèi)皮細(xì)胞顯微鏡下形態(tài)如同鵝卵石，用vWF/Factor VIII 和 CD-31容易染色,可以攝入乙?；兔芏戎鞍?。用IL-1或TNF-α預(yù)處理后，可選擇性地表達(dá)E-selectin和VCAM。
Sciencell研究實(shí)驗(yàn)室的臍靜脈內(nèi)皮細(xì)胞是從人臍帶組織分離得到的，原代凍存，冰凍運(yùn)輸。每管細(xì)胞密度*過5×10^5/ml。細(xì)胞特性經(jīng)vWF/Factor VIII和CD31 (P-CAM) 免疫熒光鑒定，能夠攝取DiI標(biāo)記乙?；兔芏戎鞍?DiI-Ac-LDL)。本細(xì)胞經(jīng)檢測不含HIV-1、HBV、HCV、支原體、細(xì)菌、酵母菌和真菌。如采用ScienCell 實(shí)驗(yàn)室特制的培養(yǎng)基，可保證此細(xì)胞15倍增增殖。
推薦培養(yǎng)基：內(nèi)皮細(xì)胞培養(yǎng)基(ECM, Cat. #1001)
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貨號(hào)
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8000
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產(chǎn)地
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美國
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縮寫
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HUVEC
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規(guī)格
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5 x 10^5 /1ml
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用途
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科研
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儲(chǔ)存
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液氮
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運(yùn)輸
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干冰
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Question:
Greetings! I am the biosafety officer at our institution, and we've had a researcher order primary HUVEC cells from your company. Would you be able to let us know if you test the cultures/isolates for infectious agents or viruses and what agents you test for?
Answers:
Each cell strain tests negative for HIV, HBV and HCV. However, keep in mind that diagnostic tests are not necessarily 100% accurate, therefore, proper precautions must be taken to avoid inadvertent exposure. Always wear gloves and safety glasses when working with these materials. Never mouth pipette. We recommend following the universal procedures for handling products of human origin as the minimum precaution against contamination.
