間充質(zhì)干細(xì)胞是特征明確的一類**干細(xì)胞。它們有發(fā)育為成熟細(xì)胞的潛在能力而產(chǎn)生脂肪、軟骨、骨骼和肌肉。這些特性與它們發(fā)育中的可塑性共同作用，使人們對(duì)用間充質(zhì)干細(xì)胞來替換受損組織的潛在能力產(chǎn)生了極大的興趣。間充質(zhì)干細(xì)胞無血清培養(yǎng)，在轉(zhuǎn)化生長(zhǎng)因子作用下會(huì)分化成軟骨細(xì)胞。相反，在有血清、抗壞血酸和地塞米松的作用下會(huì)分化成成骨細(xì)胞。間充質(zhì)干細(xì)胞有能力更新和分化成多種間充質(zhì)組織。間充質(zhì)干細(xì)胞有潛能被移植到受損傷的部位或者種植到仿生支架上，生長(zhǎng)形成適當(dāng)?shù)慕M織結(jié)構(gòu)。
Sciencell研究實(shí)驗(yàn)室的人骨髓間充質(zhì)干細(xì)胞是從人骨髓組織中分離得到的，原代凍存，冰凍運(yùn)輸。每管細(xì)胞密度*過5×10^5/ml。人骨髓間充質(zhì)干細(xì)胞經(jīng)免疫熒光鑒定、和CD73，CD90，CD105的抗體鑒定，以及分化后的脂質(zhì)染色鑒定。本細(xì)胞經(jīng)檢測(cè)不含HIV-1、HBV、HCV、支原體、細(xì)菌、酵母菌和真菌。如采用ScienCell 實(shí)驗(yàn)室特制的培養(yǎng)基，可保證此細(xì)胞15倍增。
推薦培養(yǎng)基：間充質(zhì)干細(xì)胞培養(yǎng)基?(MSCM, Cat. #7501)
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貨號(hào)
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7500
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產(chǎn)地
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美國(guó)
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縮寫
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HMSC-ad
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規(guī)格
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5 x 10^5 /1ml
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用途
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科研
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儲(chǔ)存
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液氮
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運(yùn)輸
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干冰
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1.) Lee HK, Finniss S, Cazacu S, Bucris E, Ziv-Av A, Xiang C, Bobbitt K, Rempel SA, Hasselbach L, Mikkelsen T, Slavin S, Brodie C. (2013) "Mesenchymal stem cells deliver synthetic microRNA mimics to glioma cells and glioma stem cells and inhibit their cell migration and self-renewal."?Oncotarget. 4: 346-61.
2.) Lu L, Song HF, Zhang WG, Liu XQ, Zhu Q, Cheng XL, Yang GJ, Li A, Xiao ZC. (2012) "Potential role of 20S proteasome in maintaining stem cell integrity of human bone marrow stromal cells in prolonged culture expansion."?Biochem Biophys Res Commun. 422: 121-7.
3.) Robin TP, Smith A, McKinsey E, Reaves L, Jedlicka P, Ford HL. (2012) "EWS/FLI1 regulates EYA3 in Ewing sarcoma via modulation of miRNA-708, resulting in increased cell survival and chemoresistance."?Mol Cancer Res. 10: 1098-108.?
4.) Suh N, Paul S, Lee HJ, Yoon T, Shah N, Son AI, Reddi AH, Medici D, S**** MB. (2012) "Synthetic triterpenoids, CDDO-Imidazolide and CDDO-Ethyl amide, induce chondrogenesis."?Osteoarthritis Cartilage. 20: 446-50.
5.) Tan F, Naciri M, Dowling D, Al-Rubeai M. (2012) "In vitro and in vivo bioactivity of CoBlast hydroxyapatite coating and the effect of impaction on its osteoconductivity."Biotechnol Adv. 30: 352-62.
6.) Dissanayaka WL, Zhu X, Zhang C, Jin L. (2011) "Characterization of dental pulp stem cells isolated from canine premolars."?J Endod. 37: 1074-80.
7.) Liu S, Ginestier C, Ou SJ, Clouthier SG, Patel SH, Monville F, Korkaya H, Heath A, Dutcher J, Kleer CG, Jung Y, Dontu G, Taichman R, Wicha MS. (2011) "Breast cancer stem cells are regulated by mesenchymal stem cells through cytokine networks."?Cancer Res. 71: 614-24.
8.) Liu Y, Xu X, Ma X, Martin-Rendon E, Watt S, Cui Z. (2010) "Cryopreservation of human bone marrow-derived mesenchymal stem cells with reduced dimethylsulfoxide and well-defined freezing solutions."?Biotechnol Prog. 26: 1635-43.
9.) Chadipiralla K, Yochim JM, Bahuleyan B, Huang CY, Garcia-Godoy F, Murray PE, Stelnicki EJ. (2010) "Osteogenic differentiation of stem cells derived from human periodontal ligaments and pulp of human exfoliated deciduous teeth."?Cell Tissue Res. 340: 323-33.
10.) Li GC, Ye QH, Xue YH, Sun HJ, Zhou HJ, Ren N, Jia HL, Shi J, Wu JC, Dai C, Dong QZ, Qin LX. (2010) "Human mesenchymal stem cells inhibit metastasis of a hepatocellular carcinoma model using the MHCC97-H cell line."?Cancer Sci. 101: 2546-53.
11.) Li M, Jayandharan GR, Li B, Ling C, Ma W, Srivastava A, Zhong L. (2010) "High-efficiency transduction of fibroblasts and mesenchymal stem cells by tyrosine-mutant AAV2 vectors for their potential use in cellular therapy."?Hum Gene Ther. 21: 1527-43.
12.) Medici D, Shore EM, Lounev VY, Kaplan FS, Kalluri R, Olsen BR. (2010) "Conversion of vascular endothelial cells into multipotent stem-like cells."?Nat Med. 16: 1400-6.
