成纖維細(xì)胞是來(lái)源于中胚層的間充質(zhì)細(xì)胞。乳腺的細(xì)胞外基質(zhì)大部分為乳腺成纖維細(xì)胞（MF）所分泌。更重要的是這些細(xì)胞外基質(zhì)通過(guò)影響基因表達(dá)，調(diào)節(jié)乳腺上皮細(xì)胞的增殖和分化。另外，有研究表明乳腺成纖維細(xì)胞能招募巨噬細(xì)胞的免疫監(jiān)視，這與細(xì)胞外基質(zhì)產(chǎn)生、新生血管生成等共同作用，形成乳腺微環(huán)境，促進(jìn)腫瘤細(xì)胞的發(fā)展。這些結(jié)果表明，MF能夠刺激乳腺癌的發(fā)展和侵襲。
Sciencell研究實(shí)驗(yàn)室的MF是從人乳腺分離得到的，原代凍存，冰凍運(yùn)輸。每管細(xì)胞密度*過(guò)5×10^5/ml。細(xì)胞特性經(jīng)纖維粘連蛋白CD90、vimentin免疫熒光鑒定。本細(xì)胞經(jīng)檢測(cè)不含HIV-1、HBV、HCV、支原體、細(xì)菌、酵母菌和真菌。如采用ScienCell 實(shí)驗(yàn)室特制的培養(yǎng)基，可保證此細(xì)胞15倍增增殖。
推薦培養(yǎng)基：成纖維細(xì)胞培養(yǎng)基 (FM, Cat. #2301)
Question:
Dear Sir, I would like to ask few questions. 1. What is the maximum passage number I can subculture the cells to, such that there is no significant genotypic or phenotypic change compared to cells at passage 0 as I received initially? 2. Is there a possibility to mix the cells from 3 donors into one vial with total cells of let say about 1 million cells/vial? Thank you very much for your time and attention. Would like to hear from you. Best regards, Aurino
Answers:
-
As stated in the product sheet, Human Mammary Fibroblasts are guaranteed to expand for 15 population doublings under the conditions provided. Please note that population doubling is not the same as passage number – for help, please see our Frequently Asked Questions section under Technical Resources. We do not have quantitative data measuring phenotypic or genetic changes over passage number. Unfortunately, each lot of cells is derived from one donor, so we cannot provide one vial of cells pooled from multiple donors. However, customers can request vials from different donors when purchasing more than one vial.
