小梁網(wǎng)位于虹膜底部周圍，并有一個開口進入鞏膜靜脈竇，將廢棄物質(zhì)傳輸至靜脈血管。小梁網(wǎng)細胞在房水的流出中起重要作用，且這條路徑是眼壓依賴的。小梁網(wǎng)細胞的損傷和死亡與開角青光眼的發(fā)病有關(guān)。小梁網(wǎng)細胞進一步的表達神經(jīng)遞質(zhì)和神經(jīng)肽受體，受極低濃度的血管活性多肽和生長因子調(diào)節(jié)，使這些細胞對小梁網(wǎng)的多層次滲透。體外培養(yǎng)TMC是研究小梁網(wǎng)調(diào)節(jié)功能的良好模型，并對青光眼治療技術(shù)的開發(fā)非常有幫助。
Sciencel的原代凍存，冰凍運輸。每管細胞密度*過5×10^5/ml。細胞特性經(jīng)α-平滑肌肌動蛋白和纖維粘連蛋白免疫熒光鑒定。本細胞經(jīng)檢測不含HIV-1、HBV、HCV、支原體、細菌、酵母菌和真菌。如采用ScienCell實驗室提供的實驗條件，可保證此細胞15倍增增殖。
推薦培養(yǎng)基：小梁網(wǎng)細胞培養(yǎng)基 (TMCM, Cat. #6591)
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貨號
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6590
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產(chǎn)地
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美國
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縮寫
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HTMC
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規(guī)格
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5 x 10^5 /1ml
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用途
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科研
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儲存
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液氮
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運輸
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干冰
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1.) Diskin S, Chen WS, Cao Z, Gyawali S, Gong H, Soza A, Gonz?lez A, Panjwani N. (2012) "Galectin-8 promotes cytoskeletal rearrangement in trabecular meshwork cells through activation of Rho signaling." PLoS One. 7: e44400.
2.) Famili A, Ammar DA, Kahook MY. (2013) "Ethyl pyruvate treatment mitigates oxidative stress damage in cultured trabecular meshwork cells." Mol Vis. 19: 1304-9.
3.) Sacc? SC, La Maestra S, Micale RT, Larghero P, Travaini G, Baluce B, Izzotti A. (2011) "Ability of dorzolamide hydrochloride and timolol maleate to target mitochondria in glaucoma therapy." Arch Ophthalmol. 129: 48-55.
4.) Miyamoto N, Izumi H, Miyamoto R, Kondo H, Tawara A, Sasaguri Y, Kohno K. (2011) "Quercetin induces the expression of peroxiredoxins 3 and 5 via the Nrf2/NRF1 transcription pathway." Invest Ophthalmol Vis Sci. 52: 1055-63.
5.) Hudson BD, H?bert TE, Kelly ME. (2010) "Physical and functional interaction between CB1 cannabinoid receptors and beta2-adrenoceptors." Br J Pharmacol. 160: 627-42.
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Question:
Hello. I would like to know how long the human trabecular meshwaork cells survive in culture? For example, 20 passages or 15 doublings? Thank you
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??Answers:
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Dear?Mijin,
According to the?product sheet, we guarantee 15 population doublings for this cell. Thanks!?
