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睫狀上皮細(xì)胞位于睫狀突后部的葉狀突出上，覆蓋基質(zhì)和血管的核心。它是由在結(jié)構(gòu)上和功能上都截然不同的兩層柱狀細(xì)胞組成。含色素的睫狀上皮細(xì)胞基底膜面向基質(zhì)，不含色素的睫狀上皮細(xì)胞基底膜鄰近房水。NPCEpiC的主要功能是產(chǎn)生房水，它們將溶質(zhì)從睫狀突基質(zhì)轉(zhuǎn)移到后室，從而形成滲透梯度使水從基質(zhì)流向房水。其他研究證明NPCEpiC合成光傳導(dǎo)所需的大分子和睫狀體罕見(jiàn)腫瘤發(fā)展所需的大分子。
Sciencell研究實(shí)驗(yàn)室的HNPCEpiC是從人睫狀體分離得到的，原代凍存，冰凍運(yùn)輸。每管細(xì)胞密度*過(guò)5×10^5/ml。細(xì)胞特性經(jīng)cytokeratine-18,and-19免疫熒光鑒定。本細(xì)胞經(jīng)檢測(cè)不含HIV-1、HBV、HCV、支原體、細(xì)菌、酵母菌和真菌。如采用ScienCell實(shí)驗(yàn)室特制的培養(yǎng)基，可保證此細(xì)胞15倍增增殖。
推薦培養(yǎng)基：上皮細(xì)胞培養(yǎng)基 (EpiCM, Cat. #4101)

貨號(hào)	6580
產(chǎn)地	美國(guó)
縮寫(xiě)	HNPCEpiC
規(guī)格	5 x 10^5 /1ml
用途	科研
儲(chǔ)存	液氮
運(yùn)輸	干冰

1.) Yamanouchi K, Tsuruga E, Oka K, Sawa Y, Ishikawa H. (2012) "Fibrillin-1 and fibrillin-2 are essential for formation of thick oxytalan fibers in human nonpigmented ciliary epithelial cells in vitro." Connect tissue res.53: 14-20.

2.) Kalariya NM, Shoeb M, Reddy AB, Zhang M, van Kuijk FJ, Ramana KV. (2010) "Prevention of endotoxin-induced uveitis in rats by plant sterol guggulsterone." Invest Ophthalmol Vis Sci. 51: 5105-13.

Answers:

• The doubling time is around 25 hours at early passage and around 40-50 hours at late passage. Cells can undergo 3-4 passages but we highly recommend that customers use the cells at early passage for experiment, as primary cells may change morphology and become senescent over time if not handled properly during subculture.

  you can check publication on the next tab or as follows:

  1.) Yamanouchi K, Tsuruga E, Oka K, Sawa Y, Ishikawa H. (2012) "Fibrillin-1 and fibrillin-2 are essential for formation of thick oxytalan fibers in human nonpigmented ciliary epithelial cells in vitro." Connect tissue res.53: 14-20.
  
  2.) Kalariya NM, Shoeb M, Reddy AB, Zhang M, van Kuijk FJ, Ramana KV. (2010) "Prevention of endotoxin-induced uveitis in rats by plant sterol guggulsterone." Invest Ophthalmol Vis Sci. 51: 5105-13.

  Thank you for your interest

  SCIENCELL TEAM