視網(wǎng)膜是位于眼睛后部的多層結構。它包括感光細胞和視網(wǎng)膜色素上皮細胞（RPEpiC）。RPEpiC位于神經(jīng)視網(wǎng)膜和脈絡膜之間，形成視網(wǎng)膜外屏障，控制著視網(wǎng)膜下腔的化學構成。人們發(fā)現(xiàn)RPEpiC內有許多特定偏振分布的細胞內蛋白質。與其他上皮細胞通常頂生面沒有基質相比，RPEpiC的頂生面胞膜是與細胞外基質相接觸的。RPEpiC在調節(jié)相鄰的感光膜周轉、維生素A的代謝、保護細胞內黑色素所致的視網(wǎng)膜光損害中起關鍵作用。RPEpiC與很多視網(wǎng)膜疾病的發(fā)病相關，如增殖性視網(wǎng)膜病變和年齡相關性黃斑變性。
Sciencell研究實驗室的HRPEpiC是從人視網(wǎng)膜分離得到的，原代凍存，冰凍運輸。每管細胞密度*過5×10^5/ml。細胞特性經(jīng)cytokeratine -18, -19和纖維粘連蛋白免疫熒光鑒定。本細胞經(jīng)檢測不含HIV-1、HBV、HCV、支原體、細菌、酵母菌和真菌。如采用ScienCell 實驗室特制的培養(yǎng)基，可保證此細胞10倍增增殖。
推薦培養(yǎng)基：上皮細胞培養(yǎng)基 (EpiCM, Cat. #4101)
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貨號
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6540
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產地
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美國
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縮寫
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HRPEpiC
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規(guī)格
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5 x 10^5 /1ml
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用途
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科研
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儲存
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液氮
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運輸
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干冰
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1.) Favret S, Binet F, Lapalme E, Leboeuf D, Carbadillo J, Rubic T, Picard E, Mawambo G, Tetreault N, Jean- Joyal S, Chemtob S, Sennlaub F, SanGiovanni JP, Guimond M,Sapieha P. (2013) "Deficiency in the metabolite receptor SUCNR1 (GPR91) leads to outer retinal lesions." Aging (Albany NY). 5: 427.
2.) Miyamoto N, Izumi H, Miyamoto R, Bin H, Kondo H, Tawara A, Sasaguri Y, Kohno K. (2011) "Transcriptional Regulation of Activating Transcription Factor 4 under Oxidative Stress in Retinal Pigment Epithelial ARPE-19/HPV-16 Cells." Invest Ophthalmol Vis Sci. 52: 1226-34.
3.) Narayan S, Prasanna G, Tchedre K, Krishnamoorthy R, Yorio T. (2010) "Thrombin-induced endothelin-1 synthesis and secretion in retinal pigment epithelial cells is rho kinase dependent." J Ocul Pharmacol Ther. 26: 389-97.
4.) Bertram KM, Baglole CJ, Phipps RP, Libby RT. (2009) "Molecular regulation of cigarette smoke induced-oxidative stress in human retinal pigment epithelial cells: implications for age-related macular degeneration." Am J Physiol Cell Physiol. 297: C1200-10.
5.) Mannermaa E, Vellonen KS, Ryh?nen T, Kokkonen K, Ranta VP, Kaarniranta K, Urtti A. (2009) "Efflux protein expression in human retinal pigment epithelium cell lines."Pharm Res. 26: 1785-91.
6.) Cheong HI, Johnson J, Cormier M, Hosseini K. (2008) "In vitro cytotoxicity of eight beta-blockers in human corneal epithelial and retinal pigment epithelial cell lines: comparison with epidermal keratinocytes and dermal fibroblasts." Toxicol In Vitro. 22: 1070-6.
7.) Swaney JS, Moreno KM, Gentile AM, Sabbadini RA, Stoller GL. (2008) "Sphingosine-1-phosphate (S1P) is a novel fibrotic mediator in the eye." Exp Eye Res. 87: 367-75.
8.) Hunt DW, Boivin WA, Fairley LA, Jovanovic MM, King DE, Salmon RA, Utting OB. (2006) "Ultraviolet B light stimulates interleukin-20 expression by human epithelial keratinocytes." Photochem Photobiol. 82: 1292-300.
Question:
Regarding Human Retinal Pigment Epithelial Cells Catalog #6540 Are the cells from a single human donor or multiple in a pool? If single donor, can one order vials from a few donors to compare? Can you provide a list of available donors and their characteristics? I have read the following paper where this product is used and they make the comment that they conducted their work with hRPEpC in multiple donors. Toxicol Sci. 2015 Mar;144(1):163-72. Retinal Toxicity, in vivo and in vitro, Associated with Inhibition of Nicotinamide Phosphoribosyltransferase.
Answers:
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Hi Elizabeth,
it is Single donor.if you are interested in multiple vials from different lots to get different donors. Please contact us at techsupport@sciencellonline.com to obtain donor information.
Thanks for your interest in our products.
SCIENCELL TEAM
