人角膜是一個獨特的組織，因其透明且無血管。角膜包含三個不同的細(xì)胞層，外層上皮細(xì)胞、中間層基質(zhì)和內(nèi)層內(nèi)皮細(xì)胞。角膜上皮細(xì)胞在先天免疫反應(yīng)中起著作用，通過感應(yīng)病原體存在并發(fā)出信號從而激活角膜防御系統(tǒng)。角膜上皮細(xì)胞還能高水平的表達(dá)乙醛脫氫酶，防止紫外線和4-羥基壬烯醛對細(xì)胞造成的傷害。角膜內(nèi)皮細(xì)胞的更新由細(xì)胞因子調(diào)控，比如表皮生長因子，它能激活上皮細(xì)胞深層的同源受體。體外培養(yǎng)角膜內(nèi)皮細(xì)胞已經(jīng)可以替代毒理學(xué)檢測中的動物實驗。它們也可以被用來研究角膜細(xì)胞的增殖、分化和死亡之間的高度集成和平衡。
Sciencell研究實驗室的HCEpiC是從人角膜分離得到的，原代凍存，冰凍運輸。每管細(xì)胞密度*過5×10^5/ml。細(xì)胞特性經(jīng)無血清培養(yǎng)基中鏡檢為鵝卵石形態(tài)，CK-18,和-19免疫熒光鑒定。本細(xì)胞經(jīng)檢測不含HIV-1、HBV、HCV、支原體、細(xì)菌、酵母菌和真菌。如采用ScienCell 實驗室特制的培養(yǎng)基，可保證此細(xì)胞10倍增增殖。
推薦培養(yǎng)基：角膜上皮細(xì)胞培養(yǎng)基 (CEpiCM, Cat. #6511)
|
貨號
|
6510
|
|
產(chǎn)地
|
美國
|
|
縮寫
|
HCEpiC
|
|
規(guī)格
|
5 x 10^5 /1ml
|
|
用途
|
科研
|
|
儲存
|
液氮
|
|
運輸
|
干冰
|
1.) Vellonen KS, H?kli M, Merezhinskaya N, Tervo T, Honkakoski P, Urtti A. (2010) "Monocarboxylate transport in human corneal epithelium and cell lines." Eur J Pharm Sci. 39: 241-7.
2.) Vellonen KS, Mannermaa E, Turner H, H?kli M, Wolosin JM, Tervo T, Honkakoski P, Urtti A. (2010) "Effluxing ABC transporters in human corneal epithelium." J Pharm Sci. 99: 1087-98.
3.) Belser JA, Wadford DA, Xu J, Katz JM, Tumpey TM. (2009) "Ocular infection of mice with influenza A (H7) viruses: a site of primary replication and spread to the respiratory tract." J Virol. 283: 7075-84.
4.) Cheong HI, Johnson J, Cormier M, Hosseini K. (2008) "In vitro cytotoxicity of eight beta-blockers in human corneal epithelial and retinal pigment epithelial cell lines: comparison with epidermal keratinocytes and dermal fibroblasts." Toxicol In Vitro. 22: 1070-6.
5.) Reichl S. (2008) "Cell culture models of the human cornea - a comparative evaluation of their usefulness to determine ocular drug absorption in-vitro." J Pharm Pharmacol. 60: 299-307.
6.) Zhang JZ, Cavet ME, Ward KW. (2008) "Anti-inflammatory effects of besifloxacin, a novel fluoroquinolone, in primary human corneal epithelial cells." Curr Eye Res. 33: 923-32.
7.) Hunt DW, Boivin WA, Fairley LA, Jovanovic MM, King DE, Salmon RA, Utting OB. (2006) "Ultraviolet B light stimulates interleukin-20 expression by human epithelial keratinocytes." Photochem Photobiol. 82: 1292-300.
There are no product questions yet.
