肝臟星形細(xì)胞是小葉內(nèi)結(jié)締組織細(xì)胞，呈現(xiàn)肌成纖維細(xì)胞樣或脂肪細(xì)胞形態(tài)。他們參與肝臟細(xì)胞外基質(zhì)的穩(wěn)態(tài)，修復(fù)，再生，纖維化以有控制視黃醇的代謝，存儲(chǔ)和釋放。肝臟受傷后，肝臟星形細(xì)胞轉(zhuǎn)化成肌成纖維細(xì)胞樣，并且是纖維化的肝臟中 I 型膠原的主要來(lái)源。除了以上特征，已發(fā)現(xiàn)在疾病狀態(tài)和肝內(nèi)門(mén)靜脈高壓的發(fā)病過(guò)程中，肝星狀細(xì)胞通過(guò)細(xì)胞收縮調(diào)節(jié)肝臟微循環(huán)[1,2]。星形細(xì)胞的增殖、遷移和趨化因子的表達(dá)參與了肝臟炎癥和纖維化的發(fā)病過(guò)程。因此，星形細(xì)胞激活的分子調(diào)控在將來(lái)有助于治療肝纖維化，并且能減少慢性肝病患者的發(fā)病率和死亡率。
Sciencell研究實(shí)驗(yàn)室的人肝星形細(xì)胞是從人肝臟組織中分離得到的，原代凍存，冰凍運(yùn)輸。每管細(xì)胞密度*過(guò)5×10^5/ml。人臍靜脈平滑肌細(xì)胞經(jīng)desmin 和α-actin免疫熒光鑒定。本細(xì)胞經(jīng)檢測(cè)不含HIV-1、HBV、HCV、支原體、細(xì)菌、酵母菌和真菌。如采用ScienCell 實(shí)驗(yàn)室特制的培養(yǎng)基，可保證此細(xì)胞15倍增增殖。? ?
推薦培養(yǎng)基：星形細(xì)胞培養(yǎng)基?(SteCM, Cat. #5301)
|
貨號(hào)
|
5300
|
|
產(chǎn)地
|
美國(guó)
|
|
縮寫(xiě)
|
HHSteC
|
|
規(guī)格
|
5 x 10^5 /1ml
|
|
用途
|
科研
|
|
儲(chǔ)存
|
液氮
|
|
運(yùn)輸
|
干冰
|
1.) Wagner I,?Materne EM,?Brincker S,?S??bier U,?Fr?drich C,?Busek M,?Sonntag F,?Sakharov DA,?Trushkin EV,?Tonevitsky AG,?Lauster R,?Marx U. (2013) "A dynamic multi-organ-chip for long-term cultivation and substance testing proven by 3D human liver and skin tissue co-culture."?Lab Chip. doi: 10.1039/c3lc50234a
2.) Yamana D, Shimizu T, Fan Y, Miura T, Nanashima N, Yamada T, Hakamada K, Tsuchida S. (2011) "Decrease of hepatic stellate cells in rats with enhanced sensitivity to clofibrate-induced hepatocarcinogenesis."?Cancer Sci. 102: 735-41.
3.) Das A, Shergill U, Thakur L, Sinha S, Urrutia R, Mukhopadhyay D, Shah VH. (2010) "Ephrin B2/EphB4 pathway in hepatic stellate cells stimulates Erk-dependent VEGF production and sinusoidal endothelial cell recruitment."?Am J Physiol Gastrointest Liver Physiol. 298: G908-15.?
4.) Hozawa S, Nakamura T, Nakano M, Adachi M, Tanaka H, Takahashi Y, Tetsuya M, Miyata N, Soma H, Hibi T. (2008) "Induction of matrix metalloproteinase-1 gene transcription by tumour necrosis factor alpha via the p50/p50 homodimer of nuclear factor-kappa B in activated human hepatic stellate cells."?Liver Int. 28: 1418-25.?
5.) Mart?n-V?lchez S, Sanz-Cameno P, Rodr?guez-Mu?oz Y, Majano PL, Molina-Jim?nez F, L?pez-Cabrera M, Moreno-Otero R, Lara-Pezzi E. (2008) "The hepatitis B virus X protein induces paracrine activation of human hepatic stellate cells."?Hepatology. 47: 1872-83.
6.) Semela D, Das A, Langer D, Kang N, Leof E, Shah V. (2008) "Platelet-derived growth factor signaling through ephrin-b2 regulates hepatic vascular structure and function."Gastroenterology. 135: 671-9.
7.) Shimamura T, Fujisawa T, Husain SR, Kioi M, Nakajima A, Puri RK. (2008) "Novel role of IL-13 in fibrosis induced by nonalcoholic steatohepatitis and its amelioration by IL-13R-directed cytotoxin in a rat model."?J Immunol. 181: 4656-65.
8.) Che J, Chan ES, Cronstein BN. (2007) "Adenosine A2A receptor occupancy stimulates collagen expression by hepatic stellate cells via pathways involving protein kinase A, Src, and extracellular signal-regulated kinases 1/2 signaling cascade or p38 mitogen-activated protein kinase signaling pathway."?Mol Pharmacol. 72: 1626-36.
9.) Perri RE, Langer DA, Chatterjee S, Gibbons SJ, Gadgil J, Cao S, Farrugia G, Shah VH. (2006) "Defects in cGMP-PKG pathway contribute to impaired NO-dependent responses in hepatic stellate cells upon activation."?Am J Physiol Gastrointest Liver Physiol. 290: G535-42.
10.) Brown KE, Broadhurst KA, Mathahs MM, Brunt EM, Schmidt WN. (2005) "Expression of HSP47, a collagen-specific chaperone, in normal and diseased human liver."?Lab Invest. 85: 789-97.
There are no product questions yet.
