內(nèi)皮細胞有很多生理功能，而且是許多病理進程的中心。肝臟包括兩種內(nèi)皮細胞：血管內(nèi)皮細胞和肝竇內(nèi)皮細胞。肝竇內(nèi)皮細胞是微血管內(nèi)皮細胞有獨特的表型如同樹突和如同CD4+T細胞的獨特的抗原呈遞細胞的功能。因此SEC表現(xiàn)為一種新的組織存在形式，非專業(yè)的抗原呈遞細胞，其表現(xiàn)出與控制肝臟局部的免疫應(yīng)答和免疫感應(yīng)耐受性有關(guān)[1]。肝的微環(huán)境包括門靜脈成分和血竇細胞增殖而來的可溶性介質(zhì)，通過SEC嚴格控制抗原呈遞來避免由免疫介導(dǎo)的損傷。SEC表達不同于大血管內(nèi)皮細胞的特異性表面受體、有不同的形態(tài)和代謝[3]。研究表明SEC能夠動態(tài)調(diào)節(jié)細胞表面裂孔隙度，在肝臟再生時對局部環(huán)境的區(qū)域性刺激能夠快速的反應(yīng)[2]。SEC功能紊亂和結(jié)構(gòu)改變對整個肝臟有深遠的影響。
Sciencell研究實驗室的臍靜脈內(nèi)皮細胞是從人肝臟分離得到的，原代凍存，冰凍運輸。每管細胞密度*過5×10^6/ml。細胞特性經(jīng)白蛋白、cytokeratin-18 and vimentin免疫熒光鑒定。本細胞經(jīng)檢測不含HIV-1、HBV、HCV、支原體、細菌、酵母菌和真菌。HH不能擴大培養(yǎng)或者產(chǎn)期培養(yǎng)，因為它不能增殖。
推薦培養(yǎng)基：內(nèi)皮細胞培養(yǎng)基 (ECM, Cat. #1001)
|
貨號
|
5000
|
|
產(chǎn)地
|
美國
|
|
縮寫
|
HHSEC
|
|
規(guī)格
|
5 x 10^5 /1ml
|
|
用途
|
科研
|
|
儲存
|
液氮
|
|
運輸
|
干冰
|
1.) Das A, Shergill U, Thakur L, Sinha S, Urrutia R, Mukhopadhyay D, Shah VH. (2013) "Ephrin B2/EphB4 pathway in hepatic stellate cells stimulates Erk-dependent VEGF production and sinusoidal endothelial cell recruitment." Am J Physiol Gastrointest Liver Physiol. 298: G908-15
2.) Lee SJ, Park SY, Jung MY, Bae SM, Kim IS. (2011) "Mechanism for phosphatidylserine-dependent erythrophagocytosis in mouse liver." Blood. 117: 5215-23.
3.) Das A, Shergill U, Thakur L, Sinha S, Urrutia R, Mukhopadhyay D, Shah VH. (2010) "Ephrin B2/EphB4 pathway in hepatic stellate cells stimulates Erk-dependent VEGF production and sinusoidal endothelial cell recruitment." Am J Physiol Gastrointest Liver Physiol. 298: G908-15.
4.) Guilini C, Urayama K, Turkeri G, Dedeoglu DB, Kurose H, Messaddeq N, Nebigil CG. (2010) "Divergent roles of prokineticin receptors in the endothelial cells: angiogenesis and fenestration." Am J Physiol Heart Circ Physiol. 298: H844-52.
5.) Huebert RC, Vasdev MM, Shergill U, Das A, Huang BQ, Charlton MR, LaRusso NF, Shah VH. (2010) "Aquaporin-1 facilitates angiogenic invasion in the pathological neovasculature that accompanies cirrhosis." Hepatology. 52: 238-48.
6.) Kim Y, Larkin AL, Davis RM, Rajagopalan P. (2010) "The design of in vitro liver sinusoid mimics using chitosan-hyaluronic acid polyelectrolyte multilayers." Tissue Eng Part A. 16: 2731-41.
7.) Tang L, Yang J, Liu W, Tang X, Chen J, Zhao D, Wang M, Xu F, Lu Y, Liu B, Sun Q, Zhang L, He F. (2009) "Liver sinusoidal endothelial cell lectin, LSECtin, negatively regulates hepatic T-cell immune response." Gastroenterology. 137: 1498-508.e1-5.
8.) Jung MY, Park SY, Kim IS. (2007) "Stabilin-2 is involved in lymphocyte adhesion to the hepatic sinusoidal endothelium via the interaction with alphaMbeta2 integrin." J Leukoc Biol. 82: 1156-65.
9.) Nahmias Y, Casali M, Barbe L, Berthiaume F, Yarmush ML. (2006) "Liver endothelial cells promote LDL-R expression and the uptake of HCV-like particles in primary rat and human hepatocytes." Hepatology. 43: 257-65.
10.) Nahmias Y, Odde DJ. (2006) "Micropatterning of living cells by laser-guided direct writing: application to fabrication of hepatic-endothelial sinusoid-like structures." Nat Protoc. 1: 2288-96.
11.) Perri RE, Langer DA, Chatterjee S, Gibbons SJ, Gadgil J, Cao S, Farrugia G, Shah VH. (2006) "Defects in cGMP-PKG pathway contribute to impaired NO-dependent responses in hepatic stellate cells upon activation." Am J Physiol Gastrointest Liver Physiol. 290: G535-42.
There are no product questions yet.
