腎系膜細(xì)胞是血管周圍細(xì)胞，位于腎小球毛細(xì)血管袢之間的中央部位。系膜細(xì)胞有多種功能，包括合成和組裝的系膜基質(zhì)，內(nèi)吞作用和處理血漿大分子，通過(guò)系膜細(xì)胞收縮或血管活性激素的釋放[1]調(diào)控腎小球血流動(dòng)力學(xué)。系膜細(xì)胞也能合成，聚集和控制腎小球系膜基質(zhì)成分的變化。在多種腎小球疾病中，如IgA腎病、膜增生性腎小球腎炎、狼瘡性腎炎、腎小球硬化癥、糖尿病腎病等，均能檢測(cè)到腎小球系膜基質(zhì)的生產(chǎn)過(guò)剩[2，3]。這些關(guān)鍵的作用表明腎系膜細(xì)胞是研究生理和病理環(huán)境下系膜損傷和腎小球功能的理想模型。
Sciencell研究實(shí)驗(yàn)室的人腎系膜細(xì)胞是從人的腎臟組織中分離得到的，純化后凍存，冰凍運(yùn)輸。每管細(xì)胞密度*過(guò)5×10^5/ml。該細(xì)胞可通過(guò)纖連蛋白、Thy-1和平滑肌肌動(dòng)蛋白特異性抗體的免疫熒光進(jìn)行鑒定。本細(xì)胞經(jīng)檢測(cè)不含HIV-1、HBV、HCV、支原體、細(xì)菌、酵母菌和真菌。如采用ScienCell 實(shí)驗(yàn)室特制的培養(yǎng)基，可保證此細(xì)胞15倍增殖。
推薦培養(yǎng)基：腎系膜細(xì)胞培養(yǎng)基 (MCM, Cat. #4201)
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貨號(hào)
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4200
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產(chǎn)地
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美國(guó)
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縮寫(xiě)
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HRMC
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規(guī)格
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5 x 10^5/1ml
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用途
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科研
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儲(chǔ)存
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液氮
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運(yùn)輸
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干冰
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1.) Ghada AK and J Golbahar. (2013) ?High Glucose-Induced Oxidative Stress Increases the Copy Number of Mitochondrial DNA in Human Mesangial Cells.? BioMed Research International. 2013: 1155-63
2.) Kang MK, Lim SS, Lee JY, Yeo KM, Kang YH. (2013) "Anthocyanin-Rich Purple Corn Extract Inhibit Diabetes-Associated Glomerular Angiogenesis." PloS one. 8: e79823.
3.) Li J, Lim SS, Lee ES, Gong JH, Shin D, Kang IJ, Kang YH. (2011) "Isoangustone A suppresses mesangial fibrosis and inflammation in human renal mesangial cells."Exp Biol Med (Maywood). 236: 435-44.
4.) Wu F, Zhang W, Li L, Zheng F, Shao X, Zhou J, Li H. (2011) "Inhibitory effects of honokiol on lipopolysaccharide-induced cellular responses and signaling events in human renal mesangial cells." Eur J Pharmacol. 654: 117-21.
5.) Huang P, Zhang Y, Jiang T, Zeng W, Zhang N. (2010) "Role of aldose reductase in the high glucose induced expression of fibronectin in human mesangial cells." Mol Biol Rep. 37: 3017-21.
6.) Li J, Kang SW, Kim JL, Sung HY, Kwun IS, Kang YH. (2010) "Isoliquiritigenin entails blockade of TGF-beta1-SMAD signaling for retarding high glucose-induced mesangial matrix accumulation." J Agric Food Chem. 58: 3205-12.
7.) Jiang T, Qu JJ, Nishinaka T, Zhang N. (2008) "Transcription factor AP-1 regulates TGF-beta(1)-induced expression of aldose reductase in cultured human mesangial cells." Nephrology (Carlton). 13: 212-7.
8.) Mathew JT, Patni H, Chaudhary AN, Liang W, Gupta A, Chander PN, Ding G, Singhal PC. (2008) "Aldosterone induces mesangial cell apoptosis both in vivo and in vitro."Am J Physiol Renal Physiol. 295: F73-81.
9.) Drel VR, Pacher P, Stevens MJ, Obrosova IG. (2006) "Aldose reductase inhibition counteracts nitrosative stress and poly(ADP-ribose) polymerase activation in diabetic rat kidney and high-glucose-exposed human mesangial cells." Free Radic Biol Med. 40: 1454-65.
10.) Li Y, Wen X, Spataro BC, Hu K, Dai C, Liu Y. (2006) "Hepatocyte growth factor is a downstream effector that mediates the antifibrotic action of peroxisome proliferator-activated receptor-gamma agonists." J Am Soc Nephrol. 17: 54-65.
11.) Wen X, Li Y, Hu K, Dai C, Liu Y. (2005) "Hepatocyte growth factor receptor signaling mediates the anti-fibrotic action of 9-cis-retinoic acid in glomerular mesangial cells."Am J Pathol. 167: 947-57.
12.) Dai C, Liu Y. (2004) "Hepatocyte growth factor antagonizes the profibrotic action of TGF-beta1 in mesangial cells by stabilizing Smad transcriptional corepressor TGIF." J Am Soc Nephrol. 15: 1402-12.
