腎小球內(nèi)皮細(xì)胞是一種分化的微血管細(xì)胞，參與調(diào)節(jié)腎小球的濾過。它們形成濾過屏障的內(nèi)側(cè)部分，且參與腎小球的病變。腎小球內(nèi)皮細(xì)胞持續(xù)分泌生物活性分子，并可被炎癥和血栓形成所上調(diào)。嚴(yán)重腎小球缺陷導(dǎo)致的內(nèi)皮細(xì)胞損傷，可抑制局部血管生成和損傷部位的腎小球硬化。腎小球內(nèi)皮細(xì)胞的損傷可影響腎小球系膜細(xì)胞和上皮細(xì)胞，并導(dǎo)致腎臟疾病的進(jìn)展[4]。由于腎小球內(nèi)皮細(xì)胞在培養(yǎng)、克隆和增殖方面的困難，使得該細(xì)胞的生物學(xué)特性仍不為人所知。
Sciencell研究實(shí)驗(yàn)室的人腎小球內(nèi)皮細(xì)胞是從人的腎中分離得到的，純化后凍存，冰凍運(yùn)輸。每管細(xì)胞密度*過5×10^5/ml。該細(xì)胞可通過 vWF/Factor VIII 和CD31 (PECAM)特異性抗體的免疫熒光及體外微導(dǎo)管結(jié)構(gòu)的形成進(jìn)行鑒定。本細(xì)胞經(jīng)檢測(cè)不含HIV-1、HBV、HCV、支原體、細(xì)菌、酵母菌和真菌。如采用ScienCell 實(shí)驗(yàn)室特制的培養(yǎng)基，可保證此細(xì)胞15倍增殖。
推薦培養(yǎng)基：內(nèi)皮細(xì)胞培養(yǎng)基?(ECM, Cat. #1001)
|
貨號(hào)
|
4000
|
|
產(chǎn)地
|
美國
|
|
縮寫
|
HRGEC
|
|
規(guī)格
|
5 x 10^5/1ml
|
|
用途
|
科研
|
|
儲(chǔ)存
|
液氮
|
|
運(yùn)輸
|
干冰
|
1.) Guo W, Ding J, Zhang A, Dai W, Liu S, Diao Z, Wang L, Han X, Liu W. (2014) "The Inhibitory Effect of Quercetin on Asymmetric Dimethylarginine-Induced Apoptosis Is Mediated by the Endoplasmic Reticulum Stress Pathway in Glomerular Endothelial Cells."?Int j mol sci. 15: 484-503.
2.) Park SW, Kim M, Chen SW, Brown KM, D'Agati VD, Lee HT. (2010) "Sphinganine-1-phosphate protects kidney and liver after hepatic ischemia and reperfusion in mice through S1P1 receptor activation."?Lab Invest. 90: 1209-24.
3.) Zupancic ML, Frieman M, Smith D, Alvarez RA, Cummings RD, Cormack BP. (2008) "Glycan microarray analysis of Candida glabrata adhesin ligand specificity."?Mol Microbiol. 68: 547-59.?
4.) Advani A, Kelly DJ, Advani SL, Cox AJ, Thai K, Zhang Y, White KE, Gow RM, Marshall SM, Steer BM, Marsden PA, Rakoczy PE, Gilbert RE. (2007) "Role of VEGF in maintaining renal structure and function under normotensive and hypertensive conditions."?Proc Natl Acad Sci USA. 104: 14448-53.
?
