肺間質中的大多數(shù)細胞是成纖維細胞。肺成纖維細胞除與普通的成纖維細胞類似外，它們還有自身的一些特點，如具有較長的分支過程和縫隙接合。其主要的功能是產生III型膠原，彈性蛋白和肺泡隔細胞外基質中的蛋白多糖。在肺受損后，PF在肺修復和重構中有著重要作用。炎癥損傷后局部成纖維細胞的積聚對于組織的修復至關重要。纖維細胞積聚不足或者過多都能導致組織功能異常。例如，在組織低氧造成肺動脈高壓的過程中所觀察到的外膜增厚就是成纖維細胞過度增殖導致的。
人肺成纖維細胞提取于人肺組織，原代凍存。每管含有細胞數(shù)>5×105 cells/ml，此細胞通過Fibronectin免疫熒光染色驗證，經測試不含有HIV-1、HBV、HCV、支原體、細菌、酵母和真菌。細胞可以達到15倍增。
推薦培養(yǎng)基：成纖維細胞培養(yǎng)基 (FM, Cat. #2301)
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貨號
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3300
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產地
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美國
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縮寫
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HPF
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規(guī)格
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5 x 10^5 /1ml
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用途
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科研
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儲存
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液氮
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運輸
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干冰
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1.) Sarker AH, Chatterjee A, Williams M, Lin S, Havel C, Jacob III P, Boldogh I, Hazra TK, Talbot P, Hang B. (2014) 'NEIL2 Protects against Oxidative DNA Damage Induced by Sidestream Smoke in Human Cells.'
2.) Sarker AH, Chatterjee A, Williams M, Lin S, Havel C, Jacob III P, Boldogh I, Hazra TK, Talbot P, Hang B. (2014) "NEIL2 Protects against Oxidative DNA Damage Induced by Sidestream Smoke in Human Cells." PloS one. 9: e90261.
3.) Williams M, Villarreal A, Bozhilov K, Lin S, Talbot P. (2013) "Metal and silicate particles including nanoparticles are present in electronic cigarette cartomizer fluid and aerosol." PLoS One. 8: e57987.
4.) Firth AL, Yao W, Ogawa A, Madani MM, Lin GY, Yuan JX. (2010) "Multipotent mesenchymal progenitor cells are present in endarterectomized tissues from patients with chronic thromboembolic pulmonary hypertension." Am J Physiol Cell Physiol. 298: C1217-25.
5.) Wang K, Zhang S, Weber J, Baxter D, Galas DJ. (2010) 'Export of microRNAs and microRNA-protective protein by mammalian cells.'
Question:
What is the p53 status of the cell. Is it immortalized, if so how (hTert, sv40 or else)
Answers:
They are not immortalized.
