呼吸道上皮細胞是由纖毛、無纖毛和能分泌黏液的細胞按照從近端到遠端呼吸道分布的混合細胞群組成。這類細胞的各個亞型細胞各具特點，從而不僅能形成物理屏障，可有效的防止多種有毒物質，而且通過產(chǎn)生和分泌大量化學介質和細胞因子形成高度復雜的宿主防御系統(tǒng)。支氣管上皮由表面的上皮細胞和粘液腺體組成。表面的上皮細胞由基細胞，杯狀細胞和纖毛細胞三種細胞構成，形成了基底柱狀結構，并能清除粘液纖毛。用支氣管上皮細胞培養(yǎng)分化誘導纖毛表型的研究顯示，IL-4和IL-13的刺激能改變增殖、纖毛運動、黏液分泌等一系列細胞功能。支氣管上皮細胞增殖以及其它細胞過程也部分受到EGF受體信號的調節(jié)。支氣管上皮細胞的培養(yǎng)為我們研究這類細胞的功能和病理生理機制提供了一個體外模型。
人支氣管上皮細胞提取于人支氣管，原代凍存。每管含有細胞數(shù)>5×105 cells/ml，此細胞通過Cytokeratin-18, -19和 Vimentin抗體熒光免疫方法驗證，經(jīng)測試不含有HIV-1、HBV、HCV、支原體、細菌、酵母和真菌。
推薦培養(yǎng)基：支氣管上皮細胞培養(yǎng)基 (BEpiCM, Cat. #3211)
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貨號
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3210
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產(chǎn)地
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美國
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縮寫
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HBEpiC
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規(guī)格
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5 x 10^5 /1ml
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用途
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科研
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儲存
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液氮
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運輸
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干冰
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1.) Shilo K, Wu X, Sharma S, Welliver M, Duan W, Villalona-Calero M, Fukuoka J, Sif S, Baiocchi R, Hitchcock CL, Zhao W, Otterson GA. (2013) 'Cellular localization of protein arginine methyltransferase-5 correlates with grade of lung tumors.'
2.) Shilo K, Wu X, Sharma S, Welliver M, Duan W, Villalona-Calero M, Fukuoka J, Sif S, Baiocchi R, Hitchcock CL, Zhao W, Otterson GA. (2013) "Cellular localization of protein arginine methyltransferase-5 correlates with grade of lung tumors." Diagnpathol.8: 201.
3.) Jia R, Li C, McCoy JP, Deng CX, Zheng ZM. (2010) "SRp20 is a proto-oncogene critical for cell proliferation and tumor induction and maintenance." Int J Biol Sci. 6: 806-26.
4.) Jiang H, Zhu YS, Xu H, Sun Y, Li QF. (2010) "Inflammatory stimulation and hypoxia cooperatively activate HIF-1{alpha} in bronchial epithelial cells: involvement of PI3K and NF-{kappa}B." Am J Physiol Lung Cell Mol Physiol. 298: L660-9.
5.) Wong CK, Cao J, Yin YB, Lam CW. (2010) "Interleukin-17A activation on bronchial epithelium and basophils: a novel inflammatory mechanism." Eur Respir J. 35: 883-93.
6.) Crawford M, Batte K, Yu L, Wu X, Nuovo GJ, Marsh CB, Otterson GA, Nana-Sinkam SP. (2009) "MicroRNA 133B targets pro-survival molecules MCL-1 and BCL2L2 in lung cancer." Biochem Biophys Res Commun. 388: 483-9.
7.) Lleo A, Selmi C, Invernizzi P, Podda M, Coppel RL, Mackay IR, Gores GJ, Ansari AA, Van de Water J, Gershwin ME. (2009) "Apotopes and the biliary specificity of primary biliary cirrhosis." Hepatology. 49: 871-9.
8.) Crawford M, Brawner E, Batte K, Yu L, Hunter MG, Otterson GA, Nuovo G, Marsh CB, Nana-Sinkam SP. (2008) "MicroRNA-126 inhibits invasion in non-small cell lung carcinoma cell lines." Biochem Biophys Res Commun. 373: 607-12.
