結(jié)腸直腸是惡性和非惡性血液病中的主要器官。位于結(jié)腸粘膜表面的細(xì)胞形成了主要的機(jī)械屏障，用于分離機(jī)體內(nèi)部與外部環(huán)境。除了上皮細(xì)胞在離子交換中的作用外，這些細(xì)胞還可作為膜免疫系統(tǒng)中的整體部件發(fā)揮作用。在體外，人結(jié)腸上皮細(xì)胞可產(chǎn)生T細(xì)胞抗體，而在體內(nèi)，該細(xì)胞可被刺激表達(dá)II級(jí)HLA和細(xì)胞內(nèi)黏附分子。它們也可針對(duì)改變的基因表達(dá)和成長特點(diǎn)產(chǎn)生許多細(xì)胞因子。另外，正常結(jié)腸上皮細(xì)胞也可表達(dá)α3, α5, α6, β1 和 β4整合蛋白。
Sciencell研究實(shí)驗(yàn)室的人結(jié)腸上皮細(xì)胞是從人結(jié)腸中分離得到的，一代凍存，冰凍運(yùn)輸。每管細(xì)胞密度*過5×10^5 ml。該細(xì)胞可通過CK-18和CK-19特異性抗體的免疫熒光鑒定。該細(xì)胞不含HIV-1、HBV、HCV、支原體、細(xì)菌、酵母菌和真菌。如采用ScienCell 實(shí)驗(yàn)室特制的培養(yǎng)基，可保證此細(xì)胞10倍增值。
推薦培養(yǎng)基：結(jié)腸上皮細(xì)胞培養(yǎng)基 (CoEpiCM, Cat. #2951)
|
貨號(hào)
|
2950
|
|
產(chǎn)地
|
美國
|
|
縮寫
|
HCoEpiC
|
|
規(guī)格
|
5 x 10^5 /1ml
|
|
用途
|
科研
|
|
儲(chǔ)存
|
液氮
|
|
運(yùn)輸
|
干冰
|
1.) Hua H, Chen W, Shen L, Sheng Q, Teng L. (2013) 'Honokiol augments the anti-cancer effects of oxaliplatin in colon cancer cells.'
2.) Rabineau M, Kocgozlu L, Dujardin D, Senger B, Haikel Y, Voegel JC, Freund JN, Schaaf P, Lavalle P, Vautier D. (2013) 'Contribution of Soft Substrates to Malignancy and Tumor Suppression during Colon Cancer Cell Division.'
3.) Hua H, Chen W, Shen L, Sheng Q, Teng L. (2013) "Honokiol augments the anti-cancer effects of oxaliplatin in colon cancer cells." ActaBiochimBiophys Sin.45: 773-79.
4.) Rabineau M, Kocgozlu L, Dujardin D, Senger B, Haikel Y, Voegel JC, Freund JN, Schaaf P, Lavalle P, Vautier D. (2013) "Contribution of Soft Substrates to Malignancy and Tumor Suppression during Colon Cancer Cell Division." PloS One. 8: e78468.
Question:
Was this cell drived from healthy colon? Regards, Halim
Answers:
Thanks for asking! All our cells are derived from healthy tissues. Thanks!
