平滑肌收縮是胃腸蠕動(dòng)中基本的運(yùn)動(dòng)方式。腸炎時(shí)由于平滑肌特殊肌動(dòng)蛋白的增加導(dǎo)致了平滑肌層的增厚。平滑肌肌動(dòng)蛋白可能影響收縮力的產(chǎn)生，這進(jìn)一步證明了炎癥時(shí)的腸平滑肌細(xì)胞具有可塑性。研究表明人腸平滑肌細(xì)胞在IL-1beta and TNF-alpha的刺激下分泌IL-6，IL-6能顯著的誘發(fā)全身的炎癥反應(yīng)。利用人腸平滑肌細(xì)胞的培養(yǎng)，可以幫助了解收縮、增殖和胃腸道結(jié)締組織對(duì)平滑肌細(xì)胞的反應(yīng)。
人結(jié)腸平滑肌細(xì)胞(HCSMC)提取于人結(jié)腸組織，**代凍存。每管含有細(xì)胞數(shù)>5×105 cells/ml，此細(xì)胞通過(guò)對(duì)actin 和 desmin免疫熒光染色驗(yàn)證，經(jīng)測(cè)試不含有HIV-1、 HBV、HCV、支原體、細(xì)菌、酵母和真菌。細(xì)胞可以達(dá)到15倍增。
推薦培養(yǎng)基：平滑肌細(xì)胞培養(yǎng)基 (SMCM, Cat. #1101)
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貨號(hào)
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2940
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產(chǎn)地
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美國(guó)
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縮寫(xiě)
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HCoSMC
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規(guī)格
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5 x 10^5 /1ml
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用途
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科研
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儲(chǔ)存
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液氮
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運(yùn)輸
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干冰
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1.) Zhu QS, Rosenblatt K, Huang KL, Lahat G, Brobey R, Bolshakov S, Nguyen T, Ding Z, Belousov R, Bill K, Luo X, Lazar A, Dicker A, Mills GB, Hung MC, Lev D. (2011) "Vimentin is a novel AKT1 target mediating motility and invasion." Oncogene. 30: 457-70.
2.) Cen L, Hutzen B, Ball S, DeAngelis S, Chen CL, Fuchs JR, Li C, Li PK, Lin J. (2009) "New structural analogues of curcumin exhibit potent growth suppressive activity in human colorectal carcinoma cells." BMC Cancer. 9: 99.
3.) Wei YC, Li CF, Yu SC, Chou FF, Fang FM, Eng HL, Uen YH, Tian YF, Wu JM, Li SH, Huang WW, Li WM, Huang HY. (2009) "Ezrin overexpression in gastrointestinal stromal tumors: an independent adverse prognosticator associated with the non-gastric location." Mod Pathol. 22: 1351-60.
