位于微脈管系統(tǒng)的內(nèi)皮細(xì)胞在炎癥發(fā)生過程中扮演了“門衛(wèi)”的角色，它可以吸收循環(huán)至組織和炎癥病灶的炎癥因子。研究表明，人食道微血管內(nèi)皮細(xì)胞對脂多糖、細(xì)胞素和pH暴露表現(xiàn)出很強(qiáng)的炎癥免疫反應(yīng)，因此該細(xì)胞對食道炎癥起到了關(guān)鍵的調(diào)節(jié)作用。當(dāng)暴露于酸性pH中時，該細(xì)胞表達(dá)特定的蛋白，如VCAM-1，但不表達(dá)其它蛋白，如ICAM-1。這種表達(dá)方式在胃食管反流疾病引發(fā)的細(xì)胞反應(yīng)中起到了一定的作用。
Sciencell研究實(shí)驗(yàn)室的人食道微血管內(nèi)皮細(xì)胞是從人食道組織中分離得到的，一代凍存，冰凍運(yùn)輸。每管細(xì)胞密度*過5×10^5 ml。該細(xì)胞可通過VWF/Factor VIII 及 CD31 (PECAM)特異性抗體的的免疫熒光鑒定和DiI-Ac-LDL的提取鑒定。該細(xì)胞不含HIV-1、HBV、HCV、支原體、細(xì)菌、酵母菌和真菌。如采用ScienCell 實(shí)驗(yàn)室特制的培養(yǎng)基，可保證此細(xì)胞15倍增值。
推薦培養(yǎng)基：內(nèi)皮細(xì)胞培養(yǎng)基 (ECM, Cat. #1001)
|
貨號
|
2700
|
|
產(chǎn)地
|
美國
|
|
縮寫
|
HEMEC
|
|
規(guī)格
|
5 x 10^5 /1ml
|
|
用途
|
科研
|
|
儲存
|
液氮
|
|
運(yùn)輸
|
干冰
|
1.) He Y, Wang Y, Li P, Zhu S, Wang J, Zhang S. (2011) "Identification of GPX3 epigenetically silenced by CpG methylation in human esophageal squamous cell carcinoma."Dig Dis Sci. 56: 681-8.
2.) Ito T, Sato F, Kan T, Cheng Y, David S, Agarwal R, Paun BC, Jin Z, Olaru AV, Hamilton JP, Selaru FM, Yang J, Matsumura N, Shimizu K, Abraham JM, Shimada Y, Mori Y, Meltzer SJ. (2011) "Polo-like kinase 1 regulates cell proliferation and is targeted by miR-593* in esophageal cancer." Int J Cancer. 129: 2134-46.
3.) Tsuchiya S, Fujiwara T, Sato F, Shimada Y, Tanaka E, Sakai Y, Shimizu K, Tsujimoto G. (2011) "MicroRNA-210 regulates cancer cell proliferation through targeting fibroblast growth factor receptor-like 1 (FGFRL1)." J Biol Chem. 286: 420-8.
4.) Qazi A, Pal J, Maitah M, Fulciniti M, Pelluru D, Nanjappa P, Lee S, Batchu RB, Prasad M, Bryant CS, Rajput S, Gryaznov S, Beer DG, Weaver DW, Munshi NC, Goyal RK, Shammas MA. (2010) "Anticancer activity of a broccoli derivative, sulforaphane, in barrett adenocarcinoma: potential use in chemoprevention and as adjuvant in chemotherapy." Transl Oncol. 3: 389-99.
5.) Rafiee P, Nelson VM, Manley S, Wellner M, Floer M, Binion DG, Shaker R. (2009) "Effect of curcumin on acidic pH-induced expression of IL-6 and IL-8 in human esophageal epithelial cells (HET-1A): role of PKC, MAPKs, and NF-kappaB." Am J Physiol Gastrointest Liver Physiol. 296: G388-98.
6.) Alvarez H, Rojas PL, Yong KT, Ding H, Xu G, Prasad PN, Wang J, Canto M, Eshleman JR, Montgomery EA, Maitra A. (2008) "Mesothelin is a specific biomarker of invasive cancer in the Barrett-associated adenocarcinoma progression model: translational implications for diagnosis and therapy." Nanomedicine. 4: 295-301.
7.) Ito T, Shimada Y, Kan T, David S, Cheng Y, Mori Y, Agarwal R, Paun B, Jin Z, Olaru A, Hamilton JP, Yang J, Abraham JM, Meltzer SJ, Sato F. (2008) "Pituitary tumor-transforming 1 increases cell motility and promotes lymph node metastasis in esophageal squamous cell carcinoma." Cancer Res. 68: 3214-24.
8.) Shammas MA, Qazi A, Batchu RB, Bertheau RC, Wong JY, Rao MY, Prasad M, Chanda D, Ponnazhagan S, Anderson KC, Steffes CP, Munshi NC, De Vivo I, Beer DG, Gryaznov S, Weaver DW, Goyal RK. (2008) "Telomere maintenance in laser capture microdissection-purified Barrett's adenocarcinoma cells and effect of telomerase inhibition in vivo." Clin Cancer Res. 14: 4971-80.
