口腔黏膜角化細(xì)胞是口腔疾病主要屏障，保護(hù)局部細(xì)胞免受微生物和化學(xué)物質(zhì)造成的傷害。它們通過不斷產(chǎn)生或受刺激后產(chǎn)生的細(xì)胞因子，刺激參與促發(fā)炎癥反應(yīng)，這意味著它們可能通過炎癥過程潛在地參與口腔感染的調(diào)控，該過程需要不同的白細(xì)胞介素參與，如IL-1?和IL-18?？谇火つそ腔?xì)胞表達(dá)多種不同的細(xì)胞標(biāo)志物，該表達(dá)受到目標(biāo)基因轉(zhuǎn)錄過程中鈣素誘導(dǎo)變化的影響。口腔黏膜角化細(xì)胞與特征明顯的皮膚角質(zhì)細(xì)胞在結(jié)構(gòu)和功能上有很大的相似性，并且為研究角質(zhì)細(xì)胞的生物學(xué)特性、無限增殖過程、惡性轉(zhuǎn)化提供了良好的模型。
人口腔黏膜角化細(xì)胞提取于人口腔黏膜，一代凍存。每管含有細(xì)胞數(shù)>5×105 cells/ml，此細(xì)胞通過Cytokeratine-8, -18 and -19免疫熒光染色驗(yàn)證，經(jīng)測試不含有HIV-1、HBV、HCV、支原體、細(xì)菌、酵母和真菌。細(xì)胞可以達(dá)到15倍增。
推薦培養(yǎng)基：口腔角質(zhì)細(xì)胞培養(yǎng)基 (OKM, Cat. #2611)
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貨號
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2610
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產(chǎn)地
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美國
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縮寫
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HOK
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規(guī)格
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5 x 10^5 /1ml
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用途
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科研
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儲存
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液氮
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運(yùn)輸
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干冰
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Question:
I use poly-L-lysine to coat my plate to culture HOK(Human Oral Keratinocytes) cells. I also found out that we have poly-D-lysine. Can I use poly-D-lysine to coat my plate and culture HOK or other primary cells?
Answers:
Thanks for asking! Although we have not tested it, poly-D-lysine should work for these cells.
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