淋巴系統(tǒng)是免疫系統(tǒng)的重要部分，與血管系統(tǒng)共同作用來(lái)保持組織的內(nèi)環(huán)境穩(wěn)定。淋巴系統(tǒng)從組織中得到液體和大分子物質(zhì)并將其返回血液循環(huán)，因此，淋巴系統(tǒng)在調(diào)節(jié)組織中液體，蛋白質(zhì)和壓力平衡方面起著重要作用。盡管淋巴毛細(xì)管內(nèi)皮細(xì)胞與血管內(nèi)皮有許多相似的特性，但由于結(jié)構(gòu)不同，又各具特點(diǎn)。淋巴毛細(xì)管缺少壁細(xì)胞且以基底膜不完整或缺失為特征。淋巴內(nèi)皮的特征是包含大量的內(nèi)褶和細(xì)胞質(zhì)囊泡，并且具有交錯(cuò)的細(xì)胞間連接。淋巴毛細(xì)管*顯著的特征是與間質(zhì)的一體化，間質(zhì)通過(guò)細(xì)微的彈性纖維與細(xì)胞外基質(zhì)相連。
淋巴內(nèi)皮細(xì)胞提取于人淋巴結(jié)組織，**代凍存。每管含有細(xì)胞數(shù)>5×10^5cells/ml，此細(xì)胞通過(guò)vWF/Factor VIII 和CD31 (P-CAM)免疫熒光染色驗(yàn)證，經(jīng)測(cè)試不含有HIV-1、HBV、HCV、支原體、細(xì)菌、酵母和真菌。細(xì)胞可以達(dá)到15倍增。
推薦培養(yǎng)基：內(nèi)皮細(xì)胞培養(yǎng)基(ECM, Cat. #1001)
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貨號(hào)
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2500
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產(chǎn)地
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美國(guó)
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縮寫
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HLEC
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規(guī)格
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5 x 10^5 /1ml
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用途
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科研
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儲(chǔ)存
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液氮
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運(yùn)輸
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干冰
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Question:
Can you tell me the FVIII antibody used to test these cells. What company and catalogue # please.
Answers:
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Posted by Karishma Shah on Tuesday, October 18, 2016
Dear Colleen,
We use Anti-von Willebrand factor antibody (otherwise known as Factor
VIII related antigen) from Sigma-Aldrich. The catalog number is F3520.
Question:
Do these express podoplanin
Answers:
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Posted by Manoj Sharma on Thursday, January 14, 2016
Our quality control department does not test for podoplanin
expression in Human Lymphatic Endothelial Cells. We do test for
endothelial cell markers CD-31 and Factor VIII.
